硫辛酸改善高脂小鼠氧化应激和慢性炎症的研究  

Study on the effect of LA attenuating oxidative stress and chronic inflammation in high fat diet in C57BL/6 mice

作　　者：崔珏[1] 肖瀛[1] 王斌[1] 施用晖[1] 乐国伟[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,无锡214122

出　　处：《中国免疫学杂志》2010年第7期583-586,590,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(No.30571347)

摘　　要：目的:探讨由长期喂饲高脂引发的小鼠机体氧化应激对细胞因子的分泌以及炎症相关基因表达的影响,评价通过添加抗氧化剂硫辛酸对长期氧化应激和慢性炎症的改善作用。方法:30只C57BL/6雄性小鼠,随机分为3组:对照组,高脂模型组(HFD),硫辛酸组(LA)。喂饲10周后测定血浆和脾脏的氧化应激指标。酶联免疫法检测血浆中IFN-γ、IL-4、IL-6、TNF-α、IL-10的含量。荧光定量PCR检测脾脏炎症相关基因C-Jun、NF-κB以及与抗氧化相关基因Mt-1的表达。结果:长期高脂喂饲可导致小鼠免疫器官抗氧化酶活性显著降低,MDA含量显著升高,形成氧化应激。机体长期处于氧化应激态可使血浆IL-4和IL-10显著降低,IFN-γ、IL-6和TNF-α水平显著升高,同时C-Jun和NF-κB炎症反应相关基因表达上调、Mt-1的表达水平下调。添加0.1%LA可有效地防止机体氧化应激的形成。结论:长期高脂喂饲导致的氧化应激可影响机体的免疫功能并诱发慢性炎症反应,LA通过直接清除自由基、恢复氧化还原平衡解除慢性氧化应激从而保护小鼠的免疫功能免受损伤。Objective：To explore the effect of lipoic acid （LA） on chronic oxidative stress,cytokines and inflammatory gene expression with mice fed with high fat diet （HFD） and whether LA supplementation could prevent development of chronic inflammation.Methods：C57BL/6 mice were randomly assigned to three groups.The control group were administrated with an ordinary diet.The two experimental groups were fed with a high fat diet or high fat plus 0.1% LA.Antioxidants defense index such as SOD,CAT,GSH-Px and MDA were examined after 10 week.Cytokines such as IFN-γ,IL-4,IL-6,TNF-α and IL-10 were examined after 10 week,respectively.Gene expression related to oxidative stress and inflammation were confirmed by QRT-PCR.Results：HFD led to potently weaken antioxidant defenses in mice.HFD significantly increased levels of IFN-γ,IL-6 and TNF-α,and decreased levels of IL-4 and IL-10 in mice plasma.QRT-PCR results showed an up-regulation of inflammation related genes and a down-regulation antioxidant-related genes.Conclusion：LA is a possibly effective supplementation with HFD,both to prevent from the development of long-term oxidative stress and to attenuate chronic inflammation.

关 键 词：硫辛酸 高脂日粮 氧化应激 慢性炎症反应 

分 类 号：R392[医药卫生—免疫学]