外周血纯真、记忆性CD8^+T细胞数量与中国HIV/AIDS患者疾病进程的相关性研究  

Study on the correlation between the number alterations of na■ve and memory CD8^+T cell subsets and disease progression in Chinese HIV/AIDS patients

作　　者：施万英[1] 张子宁[1] 张旻[1] 刘静[1] 孙国权[1] 王亚男[1] 韩晓旭[1] 姜拥军[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中国免疫学杂志》2010年第7期655-658,F0003,共5页Chinese Journal of Immunology

基　　金：国家科技重大专项课题(2008ZX10001-001);国家重点基础研究发展计划973计划(2006CB504206);辽宁省教育厅高等学校创新团队项目(2006T134);辽宁省医学重点专科项目(辽卫函字【2007】499号)资助

摘　　要：目的:研究不同疾病进展阶段的中国HIV/AIDS患者外周血CD8+T细胞亚群的数量变化,以探讨各亚群与HIV感染疾病进展的相关性。方法:首先应用多参数(CD45RA、CCR7、CD28)流式细胞术圈定幼稚(nave)CD8+T细胞和记忆性(memory)CD8+T细胞(包括TCM、TEM-1及TEM-2),随后测定58例不同疾病进展阶段HIV/AIDS患者和20例健康对照者外周血各CD8+T细胞亚群的绝对数量和占CD8+T细胞的百分比,分析其与病毒载量、CD4+T细胞数量的相关性。结果:HIV/AIDS患者与健康对照相比,nave CD8+T细胞百分比和绝对值均显著减少,memory CD8+T细胞百分比和绝对值均显著升高(P<0.05)。在memoryCD8+T细胞各亚群中,效应型(effector memory T cells,TEM)-1百分比显著升高(P<0.05)。HIV感染缓慢进展(slow progressor,SP)组、HIV组及AIDS组中,nave CD8+T细胞百分比和绝对值均依次下降,AIDS组、HIV组显著低于SP组(P<0.05),memory CD8+T细胞绝对值依次下降,各组间的下降均有显著性差异(P<0.05)。在memory CD8+T细胞各亚群中,TEM-1百分比依次升高,AIDS组显著高于SP组(P<0.05);TEM-2百分比及绝对值依次下降,AIDS组显著低于SP组(P<0.05)。相关性分析发现,nave CD8+T细胞与CD4+T细胞显著正相关(P<0.01),与HIV病毒载量显著负相关(P<0.01);中央型(central memory T cells,TCM)和TEM-2型memory CD8+T细胞与CD4+T细胞显著正相关(P<0.01)。结论:Nave、memory CD8+T细胞数量与HIV疾病进展显著相关。Objective：To characterize the alterations of CD8^＋T cells and their subsets in different stages of Chinese HIV/AIDS patients,and to investigate the correlation between the parameters and HIV disease progression.Methods：Flow cytometry was used to analyze the frequency of naive CD8^＋T cells and the three subsets of memory CD8^＋T cells：TCM,TEM-1 and TEM-2 in different stages of HIV/AIDS progression.Results：Compared with those of healthy controls,the percentages and absolute numbers of naive CD8＋T cells significantly decreased,but memory CD8^＋T cells significantly increased in Chinese HIV/AIDS patients.Of memory CD8^＋T subsets,the percentage of TEM-1 significantly increased （P〈0.05）.In SP,HIV,and AIDS groups,the percentages and absolute numbers of naive CD8^＋T cells continuously decreased.AIDS and HIV groups were significantly lower than SP group （P〈0.05）,and the absolute numbers of memory CD8＋T cells decreased.There were significant differences among each group （P0.05）.Of memory CD8^＋T subsets,the percentages of TEM-1 continuously increased,and AIDS group was significantly higher than SP group （P〈0.05）.The percentages and absolute numbers of TEM-2 continuously decreased,and AIDS group was significantly lower than SP group （P〈0.05）.The number of naive CD8＋T cells was correlated with CD4^＋T cell number （P〈0.01） and negatively correlated with viral load （P〈0.01）.Numbers of CD8^＋T TCM and TEM-2 were correlated with CD4^＋T cell number （P〈0.01）,but have no relationships with viral load.Conclusion：Numbers of naive and memory CD8^＋T cells are correlated with HIV disease progression.

关 键 词：人类免疫缺陷疾病 艾滋病 纯真细胞 记忆细胞 CD8+T细胞 

分 类 号：R392.9[医药卫生—免疫学]