柽柳甘油醛-3-磷酸脱氢酶基因的克隆与表达分析  

Cloning and Expression of a Glyceraldehyde-3-phosphate Dehydrogenase Gene from Tamarix hispida

作　　者：于丽丽[1] 高彩球[1] 王玉成[1] 姚启超[1] 刘桂丰[1] 杨传平[1] 刘燕[2] 

机构地区：[1]林木遗传育种与生物技术教育部重点实验室(东北林业大学),哈尔滨150040 [2]吉林省集安市农业局

出　　处：《东北林业大学学报》2010年第7期105-108,共4页Journal of Northeast Forestry University

基　　金：东北林业大学研究生科技创新项目;中央高校基本科研业务费专项资金(DL09BA06)资助

摘　　要：从柽柳(Tamarix hispida)cDNA文库中分离出甘油醛-3-磷酸脱氢酶基因(ThGAPDH)全长cDNA序列,该基因全长1294bp。其中:5′非翻译区84bp,3′非翻译区184bp,开放阅读框1206bp,编码含341个氨基酸的蛋白质,编码蛋白的相对分子质量为37200,理论等电点(pI)为6.97。采用实时定量RT-PCR方法研究了刚毛柽柳在PEG、NaCl、NaHCO3及CdCl2胁迫下不同时间该基因ThGAPDH的表达模式。结果表明:PEG、NaCl及CdCl2处理均能诱导ThGAPDH基因在根中的表达而抑制其在茎和叶中的表达,而NaHCO3处理均能诱导该基因在根、茎、叶中的表达。基因ThGAPDH的cDNA序列在GenBank中的登录号为GQ478708。The full length cDNA of a glyceraldehyde-3-phosphate dehydrogenase （ThGAPDH） gene was cloned from a cDNA library of Tamarix hispida. The cloned ThGAPDH gene is 1 294 bp in length,which includes a 84 bp of 5＇ untranslated region（UTR） and a 184 bp of 3＇ untranslated region. The ThGAPDH has an open reading frame（ORF） of 1 206 bp in length,encoding a protein of 341 amino acid residues with the molecular mass of 37.2 kDa and theoretical isoelectric point of 6.97. The expression of ThGAPDH gene in T. hispida responding to PEG,NaCl,NaHCO3 and CdCl2 was further investigated using real time quantitative RT-PCR. The expression of ThGAPDH was induced by PEG,NaCl and CdCl2 in roots of T.hispida,but inhibited in both stems and leaves. However,the expression of ThGAPDH was induced by NaHCO3 in roots,stems and leaves. The ThGAPDH was accepted by GenBank and the accession number is GQ478708.

关 键 词：刚毛柽柳 甘油醛-3-磷酸脱氢酶 实时定量RT-PCR 基因表达 

分 类 号：Q943.2[生物学—植物学]