刺槐心材腐朽菌的鉴定及其生物学特性  

Identification and Biological Characteristics of Locust Heartwood Rot Fungi

作　　者：高国平[1] 信雯[1] 郑雅楠[1] 高拓[1] 祁金玉[1] 张伟[1] 魏振宏[1] 

机构地区：[1]沈阳农业大学,沈阳110161

出　　处：《东北林业大学学报》2010年第8期60-62,共3页Journal of Northeast Forestry University

摘　　要：通过形态学和分子生物学方法鉴定沈阳地区刺槐心材腐朽病病原菌(编号为P1),并在室内培养条件下,采用生长速率法研究了温度、pH值、光照、碳源和氮源对病原菌菌丝生长和菌落形态的影响。结果表明:病原菌P1为槐生多年卧孔菌(Perenniporia robiniophila(Murr.)Ryv.);菌丝生长适宜温度为20～25℃,适宜pH值为5～6,光照对菌丝生长无显著影响。此外,P1对碳源的利用以蔗糖为最佳,氮源以酵母膏最佳。Heartwood rot fungus （ P1 ） of Robinia pseudoacacia in Shenyang was identified by morphologacal and molecular bio- logical methods. And the effects of temperature, pH value, light, and carbon and nitrogen resources on mycelium growth and colony morphology were studied by growth rate method. Results showed that P1 was identified as Perenniporia robinio- phil（ Murr. ） Ryv. The optimum temperature for the mycelium growth was 20 - 25 degrees C, and the optimum pH value was within the range of 5 - 6. Illumination had no obvious effect on mycelium growth. The mycelium grew best on media with cane sugar as carbon source or yeast extract as nitrogen resources.

关 键 词：刺槐 病原菌鉴定 生物学特性 ITS RDNA 

分 类 号：S792.26[农业科学—林木遗传育种]