生物酶连续消化法对犬颈总动脉脱细胞基质材料生物力学的影响研究  

Effects of decellularization using biotic enzymes on the mechanical properties of the canine carotid artery

作　　者：刘国锋[1] 何志娟[2] 杨大平[1] 徐学武[1] 刘莺[1] 任丽虹[1] 李庆春[1] 

机构地区：[1]哈尔滨医科大学附属第二医院整形外科,黑龙江哈尔滨150086 [2]哈尔滨医科大学附属第一医院妇产科

出　　处：《中国美容医学》2010年第7期996-999,共4页Chinese Journal of Aesthetic Medicine

基　　金：中国博士后科学基金项目资助(20100471098);黑龙江省青年科学基金项目资助(QC2009C38);哈尔滨市科技创新人才研究专项基金项目资助(2010RFQXS081);黑龙江省卫生厅科科研基金项目资助(2009-124);哈尔滨医科大学附属第二医院院博士科研基金项目资助(BS2009-18)

摘　　要：目的:探讨利用生物酶连续消化法制备犬颈总动脉脱细胞血管基质时对材料生物力学的影响。方法:利用胰酶和核酸酶连续消化法制备犬颈总动脉脱细胞血管基质材料,通过组织学、扫描电镜观察和DNA含量的检测,判断细胞的脱除情况;检测脱细胞材料的拉伸强度、爆裂强度及顺应性,判断脱细胞过程对材料生物力学性质的影响。结果:组织学染色及扫描电镜的结果表明血管细胞成分被完全去除,血管细胞外基质成分保留完整;与新鲜犬颈总动脉相比,脱细胞材料的拉伸强度和爆裂强度没有明显的改变,而材料的顺应性有所降低。结论:生物酶连续消化法制备的犬颈总动脉脱细胞基质材料保持了与新鲜血管大部分生物力学性质,但材料的顺应性有所降低。Objective The objective of this study is to investigate the effects of decellularization using biotic enzymes on the mechanical and structural properties of the canine carotid artery.Methods Intact canine carotid artery were decellularized by using Trypsin/EDTA,ribonuclease and desoxyribonuclease.Residual cellular and extracellular matrix composition was evaluated with hematoxylin and eosin （HE） staining,quantitative DNA analysis and scanning electron microscopy.Tensile strength,burst strength and compliance were measured in vitro to determine the effects of decellularized process on the biomechanical properties of the canine carotid artery.Results Histology and scanning electron microscopy examination demonstrate that scaffolds were completely decellularized and scaffolds revealed a well-preserved extracellular matrix.Compared with fresh canine carotid artery,decellularized artery had similar burst and breaking strength and had lower compliance.Conclusion This study demonstrates that the decellularized artery using biotic enzymes had similar burst and breaking strength and had lower compliance compared with fresh canine carotid artery.

关 键 词：血管 组织工程 细胞外基质 生物力学 

分 类 号：R318[医药卫生—生物医学工程]