吴茱萸中吴茱萸次碱与吴茱萸碱含量的超临界流体色谱法测定  

Determination of Rutaecarpine and Evodiamine in Evodia Rutaecarpa by Supercritical Fluid Chromatography

作　　者：杨敏[1] 周萍[1] 徐路[1] 马银海[2] 郭亚东[3] 

机构地区：[1]大理学院药学院,云南大理671000 [2]昆明学院化学系,云南昆明650031 [3]昆明医学院药学院,云南昆明650031

出　　处：《分析测试学报》2010年第7期743-746,共4页Journal of Instrumental Analysis

基　　金：云南省教育厅科研基金资助项目(08Y0364)

摘　　要：建立了超临界流体色谱法分离测定吴茱萸中吴茱萸次碱和吴茱萸碱含量的方法,并研究了其影响因素。使用Kromasil Slica填充柱,流动相为含13%甲醇的CO2,流速2.0 mL.min-1,柱温60℃,背压2.0×107Pa,检测波长225 nm,测得吴茱萸次碱和吴茱萸碱的线性范围分别为0.33~8.25μg（r=0.999 3）、0.68~17μg（r=0.999 8）,平均加标回收率均为100%。与传统方法相比,超临界流体色谱可在简单的流动相条件下对吴茱萸中的吴茱萸次碱和吴茱萸碱进行良好分离。A supercritical fluid chromatographic（SFC） method for the determination of rutaecarpine and evodiamine in Evodia rutaecarpa was established.The separation of rutaecarpine and evodiamine was performed on a Kromasil Slica column（250 mm×4.6 mm,5 μm） using CO2 containing 13% methanol as mobile phase at a flow rate of 2.0 mL·min-1.The back pressure was set at 2.0×107 Pa and the column temperature was 60 ℃.The detection wavelength was 225 nm.The result indicated that the linear ranges of rutaecarpine and evodiamine were in the range of 0.33-8.25 μg（r=0.999 3） and 0.68-17 μg（r= 0.999 8）,respectively.The average recoveries were both 100% with RSD of 2.6% for rutaecarpine and 3.1% for evodiamine,respectively.Comparing with the traditional method,the proposed method was simple,rapid and sensitive,and could be successfully applied in the separation of rutaecarpine and evodiamine.

关 键 词：超临界流体色谱 吴茱萸 吴茱萸次碱 吴茱萸碱 

分 类 号：O657.72[理学—分析化学]