抗草甘膦转基因大豆(RRS)对根际微生物和土壤氮素转化的影响  

Effects of Roundup Ready Soybean (RRS) on Microorganisms and Nitrogen Transformation in the Rhizospheric Soil

作　　者：刘佳[1] 刘志华[1] 徐广惠[1] 王宏燕[1] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《农业环境科学学报》2010年第7期1341-1345,共5页Journal of Agro-Environment Science

基　　金：黑龙江省自然科学基金(04-0402:ZJN)

摘　　要：采用室外小区试验方法,研究了抗草甘膦转基因大豆(RRS)对根际土壤细菌、真菌、放线菌、氨化细菌、硝化细菌和反硝化细菌的氨化作用强度、硝化作用强度和反硝化作用强度的影响。结果显示,RRS显著降低了根际土壤细菌和放线菌的数量,提高了根际土壤真菌的数量;RRS根际土壤氨化细菌、硝化细菌和反硝化细菌低于其亲本非转基因大豆和部分栽培大豆;RRS对根际土壤氨化作用强度和硝化作用强度有显著影响,但对反硝化作用强度的影响不显著(P<0.05)。研究表明,RRS不同程度上降低了根际土壤微生物的数量和生化强度,并对根际土壤真菌生长有一定的促进作用。Roundup Ready Soybean（RRS）is one of genetically modified crops and the majority of study on it address only potential risks and aboveground effects.There is little attention paid on the effect of genetically modified crops on microbial communities and biochemical reac-tions in soil.This experiment studied the effect of RRS on bacteria,fungi,actinomycete,ammonification bacteria,nitrification bacteria,den-itrification bacteria and intensities of ammonification and nitrification.Results showed that RRS reduced the quantity of bacteria and actino-mycete but increased the quantity of fungi in the rhizospheric soil.The amount of ammonification bacteria,nitrification bacteria and denitrifi-cation bacteria were all lower in the RRS rhizospheric soil than those of non-genetically modified and other cultivated soybean cultivars.RRS had a significant effect on the intensity of ammonification and nitrification,but its effect on denitrification intensity was not significant（P 0.05）in the rhizospheric soil.In general,RRS had a negative effect on the amount of bacteria and biochemical intensities to some degree but a positive effect on fungi in the rhizospheric soil.

关 键 词：抗草甘膦转基因大豆 根际土壤 微生物 生化强度 

分 类 号：X53[环境科学与工程—环境工程]