烧伤病房铜绿假单胞菌耐药谱检测及分子流行病学研究  

Antibiotic Resistance and Molecular Epidemiology Analysis of Pseudomonas aeruginosa Isolated from Burn Wards

作　　者：冯伟云[1] 胡锡浩[1] 许小敏[1] 毛联钢[1] 

机构地区：[1]宁波市第二医院临床研究室,浙江宁波315010

出　　处：《中华医院感染学杂志》2010年第14期2009-2011,共3页Chinese Journal of Nosocomiology

基　　金：宁波市自然科学基金(2008A610090)

摘　　要：目的了解分离自烧伤病房铜绿假单胞菌耐药表型及分子流行病学特性。方法对分离自烧伤病房的32株铜绿假单胞菌采用K-B法测定抗菌药物的敏感性;利用碱裂解法提取质粒获得质粒DNA图谱;利用脉冲场电泳分析菌株的亲缘性。结果 32株铜绿假单胞菌中有17株对>8种抗菌药物耐药率,占53.1%,8株(25.0%)铜绿假单胞菌对检测抗菌药物全部耐药;29株(90.6%)含有质粒,分属于4种质粒表型,主要有2种质粒表型的铜绿假单胞菌;脉冲场电泳聚类分析显示铜绿假单胞菌感染主要由3个克隆株引起。结论分离自烧伤病房的铜绿假单胞菌耐药严重,医院感染铜绿假单胞菌可导致克隆传播,甚至暴发流行。OBJECTIVE To investigate the resistance patterns and the characterization of molecular epidemiology of Pseudomonas aeruginosa isolated from burned wards.METHODS The susceptibility of these strains to 13 kinds of antimicrobial agents was detected by K-B tests.The plasmid extraction was performed by alkaline lysis method and the plasmid pattern was obtained by agarose gel electrophoresis.RESULTS Analysis of P.aeruginosa strain was performed by pulsed field gel electrophoresis（PFGE）.There were 17 strains（53.1%） resistant to at least 8 different antibacterials from 32 isolated P.aeruginosa strains,8 strains were resistant to all 13 different antibacterials.Plasmid was detected in 29 isolates（90.6%） that produced 4 different patterns,P.aeruginosa infection in burned patients was produced principally by 2 different plasmid patterns.PFGE cluster analysis demonstrated that the P.aeruginosa infection in burned patients was produced principally by 3 different clone strains.CONCLUSIONS The multidrug resistance in P.aeruginosa is a severe issue,Hospital P.aeruginosa infection can induce clone transmitted hospital infection even with fulminant prevalence.

关 键 词：铜绿假单胞菌 耐药性 质粒图谱 脉冲场电泳 医院感染 

分 类 号：R378.991[医药卫生—病原生物学]