氨基胍对胰岛β细胞保护作用的机理研究  

作　　者：何凌[1] 何美艳[1] 余绮玲[1] 黄春苓[1] 梁伟[1] 王燕[1] 陈定宇[1] 

机构地区：[1]广州市第一人民医院内分泌科,510180

出　　处：《当代医学》2010年第18期1-3,共3页Contemporary Medicine

基　　金：广州市医药卫生科技项目(2008-YB-002;2009-YB-001);广东省医学科研课题(B2006125)

摘　　要：目的探讨氨基胍(AG)对胰岛β细胞的保护作用及机理,为胰岛β细胞的保护治疗提供实验依据。方法培养胰岛β细胞株NIT细胞,分别与以下4组作用:①(IL-1β+IFN-γ)组、②(IL-1β+IFN-γ+AG)组、③AG组、④对照组(DMEM)。硝酸还原酶法检测上清一氧化氮(NO)水平,比色法检测上清诱导型一氧化氮合酶(iNOS)水平,化学发光法检测上清胰岛素(Ins)水平。结果①AG+IL-1β+IFN-γ刺激的NIT-1细胞与IL-1β+IFN-γ刺激组相比,分泌NO、iNOS水平下降(P<0.05)。②AG+IL-1β+IFN-γ刺激的NIT-1细胞与IL-1β+IFN-γ刺激组相比,Ins分泌增加(P<0.05)。结论氨基胍可减轻IL-1β联合IFN-γ对NIT细胞的损伤作用,保护NIT细胞的胰岛素分泌功能。Objective To investigate the protective effect and mechanism of Aminoguanidine（AG） on islet β cells thus to provide experiment evidence of protective therapy on islet β cells.Methods Islet β cell line NIT cells were cultivated with the following components：①IL-1β＋IFN-γ,②IL-1β＋IFN-γ＋AG,③AG,④DMEM.Nitric oxide level in supernatants was tested with nitrate reductase assay,inducible nitric oxide synthase（iNOS） level in supernatants was tested with colorimetric technique,insulin level in supernatants were tested with chemiluminescence assay.Results ①NO and iNOS concentration in supernatants of AG＋IL-1β＋IFN-γ stimulation group were lower than IL-1β＋IFN-γ group（P0.05）.②Insulin concentration in supernatants of AG＋IL-1β＋IFN-γ stimulation group were higher than IL-1β＋IFN-γ group（P0.05）.Conclusion AG alleviates the damage of IL-1β combined with IFN-γ on NIT cells and protects insulin secretion function of NIT cells.

关 键 词：氨基胍 胰岛Β细胞 保护 

分 类 号：R587.1[医药卫生—内分泌]