Two-step fermentation process and the corresponding study on its enzymology characteristics  

作　　者：常玉广 马放 郭静波 

机构地区：[1]School of Municipal and Environmental Engineering,Harbin Institute of Technology [2]School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University

出　　处：《Journal of Harbin Institute of Technology(New Series)》2010年第3期318-322,共5页哈尔滨工业大学学报（英文版）

基　　金：Sponsored by the National Basic Research Program of China (Grant No. 2004CB418505)

摘　　要：Strain HIT-3,which has good performances in cellulose degradation,was isolated from the campus soil. Based on the identification of conservative sequences 16S rDNA and the analysis of physiological-biochemical characteristics,HIT-3 was identified as Achromobacter xylosoxidans. Denitrificans. A two-step fermentation process was conducted by adopting compound-bioflocculant-producing flora constructed by cellulose-degrading bacterium HIT-3 and flocculating bacterium F2. The cellulose degradation metabolites of HIT-3 was taken as substrates by flocculating bacterium F2,by which excellent compound bio-flocculant was obtained. In addition,the enzymology characteristics of HIT-3 were investigated when cultured in cellulose media,which utilized CMC-Na as its sole carbon. The results show that HIT-3 achieves a climax of 67.6 U/mL of enzyme production after incubation for 6 d,and the organic carbons produced are sufficient as the substrates required by the fermentation of flocculating bacterium F2(flocculating efficiency of 85.6%),which makes it feasible to reuse bioenergy.Strain HIT-3, which has good performances in cellulose degradation, was isolated from the campus soil. Based on the identification of conservative sequences 16S rDNA and the analysis of physiological-biochemical characteristics, HIT-3 was identified as Achromobacter xylosoxidans. Denitrificans. A two-step fermentation process was conducted by adopting compound-bioflocculant-producing flora constructed by cellulose-degrading bacterium HIT-3 and flocculating bacterium F2. The cellulose degradation metabolites of HIT-3 was taken as substrates by flocculating bacterium F2, by which excellent compound bio-flocculant was obtained. In addition, the enzymology characteristics of HIT-3 were investigated when cultured in cellulose media, which utilized CMC-Na as its sole carbon. The results show that HIT-3 achieves a climax of 67.6 U/mL of enzyme production after incubation for 6 d, and the organic carbons produced are sufficient as the substrates required by the fermentation of flocculating bacterium F2 （flocculating efficiency of 85.6% ）, which makes it feasible to reuse bioenergy.

关 键 词：two-stage fermentation cellulose degrading bacterium flocculating bacterium cellulase activity 

分 类 号：Q539.3[生物学—生物化学]