多成分评价优化柴芩清肝方提取工艺研究  

Optimization of Extraction Technology for Chaiqin Qinggan Formular with Multiple Components Evaluation

作　　者：付克[1] 张坤[1] 闫广利[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《中国实验方剂学杂志》2010年第7期10-12,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省科技攻关项目资助(GC08C322)

摘　　要：目的:优化柴芩清肝方的提取工艺。方法:建立柴芩清肝方中黄芩苷、甘草苷和芍药苷3种成分同时测定的HPLC方法。采用L9(34)正交设计法,以黄芩苷、甘草苷、芍药苷3种成分含量综合评分法进行数据分析,对柴芩清肝方的提取次数、提取时间和加水量3因素进行优化。结果:3因素中只有提取次数有显著影响,最佳提取工艺为A3B1C3。结论:多成分评价法优选柴芩清肝方提取工艺能较好地保证制剂的质量。Objective：To optimize the extraction technology of Chaiqin Qinggan Formular.Method：A HPLC method was established to simultaneously determine the contents of baicalin,paeoniflorin and glycyrrhizin in the extractions of Chaiqin Qinggan Formular.Based on the orthogonal design of L9（3^4） and the weighted method,three factors including the multiple water,decocting time,and extracting times were evaluated.Result：Significant effect was observed in extracting times.The best extraction process is A 3 B 1 C 3 .Conclusion：The extraction technology optimized with multiple components evaluation is reasonably practicable.

关 键 词：柴芩清肝方 正交设计 黄芩苷 芍药苷 甘草苷 

分 类 号：R283.6[医药卫生—中药学]