HPLC法测定独一味制剂中山栀苷甲酯  

Determination of Shanzhiside Methylester in Preparations of Lamiophlomis Rotata by HPLC

作　　者：何希瑞[1,2] 李茂星[2] 樊鹏程[2] 尉丽力[1,2] 张汝学[2] 贾正平[1,2] 

机构地区：[1]兰州大学,兰州730000 [2]兰州军区兰州总医院,兰州730050

出　　处：《中国实验方剂学杂志》2010年第7期50-52,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：全军中医药研发推广项目(2006032001);甘肃省科技攻关项目(2GS054-A43-014-14)

摘　　要：目的:建立独一味制剂中山栀苷甲酯的HPLC测定方法,为进一步控制独一味制剂的质量提供良好的参考。方法:采用HPLC反向色谱柱Symmetry C18(4.6mm×150mm,5μm),梯度洗脱,0～9min乙腈-水(9∶91),9～20min乙腈-水(15∶85),流速1.0mL·min-1,柱温20℃,检测波长235nm。结果:山栀苷甲酯浓度在9.6～115.2μg·mL-1范围内与峰面积积分值线性关系良好;加样回收率为97.69%。从所有的制剂中均检测出山栀苷甲酯,但含量差别较大。结论:该方法准确、简便、专属性强、重现性好,适用于独一味制剂中山栀苷甲酯的含量测定。Objective：To establish a HPLC method for the determination of shanzhiside methylester in the preparations of Lamiophlomis rotata.Method：HPLC analysis was performed on Symmetry C 18（5μm） column and gradient elution0-9 min,acetonitrile-water（9∶91） ,9-20 min,acetonitrile-water（15∶85） was used as the mobile phase at a flow rate of 1.0 ml·min^-1 .The column temperature was 20℃and the detection wavelength was at 235 nm.Result：The linear range of shanzhiside methylester was 9.6～115.2μg·mL^-1（r=0.999 8） and the average recovery was 97.69%.Shanzhiside methylester could be detected from all preparations and the content variedgreatly.Conclusion：The method can be applied to determine the content of shanzhiside methylester in the preparations of L.rotata.

关 键 词：高效液相色谱 独一味 山栀苷甲酯 含量测定 

分 类 号：R284.1[医药卫生—中药学]