益母草水苏碱对大鼠心肌细胞肥大的肌浆网钙摄取及SERCA活性的影响  

Effects on Calcium Uptake Capacity and Activity of SERCA in Rat Sarcoplasmic Reticulum of Myocardial Hypertrophy Cell of Stachydrine

作　　者：孙祝美[1] 李华[1] 吕嵘[1] 赵培[1] 

机构地区：[1]上海中医药大学基础医学院生物教研室,上海201203

出　　处：《中国实验方剂学杂志》2010年第7期118-122,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市教育委员会科研项目(08cz04);浦东新区科技发展基金创新资金项目(PKJ2009-Y14)

摘　　要：目的:探讨益母草水苏碱(stachydrine,Sta)对去甲肾上腺素(norepinephrine,NE)诱导的新生大鼠心肌细胞肥大肌浆网钙摄取及SERCA活性的影响。方法:采用不同浓度的益母草水苏碱(10-4,10-5,10-6mol·L-1)对NE诱导的心肌肥大模型组进行干预,通过大鼠心肌肌浆网的蛋白水平和钙摄取能力来反映益母草水苏碱对NE诱导的新生大鼠心肌细胞肥大的影响。结果:益母草水苏碱能够明显减少心肌细胞蛋白含量,降低Protein/DNA比值;益母草水苏碱可剂量依赖地提高NE诱导的心肌肥大细胞的肌浆网钙摄取和SERCA活性。结论:益母草水苏碱对NE诱导的心肌肥大细胞的肌浆网钙摄取功能有一定的程度的提高,与剂量有一定的依赖关系,随着益母草水苏碱的浓度的提高,肌浆网的钙摄取速率和SERCA活性也明显提高。Objective：To investigate the stachydrine（Sta） on norepinephrine（NE） -induced hypertrophy of neonatal rat cardiac myocyte sarcoplasmic reticulum calcium uptake capacity and activity of SERCA. Method：different concentrations of stachydrine（10^-4,10^-5,10^-6 mol·L^-1） on the NE-induced cardiac hypertrophy model group intervention,by rat cardiac sarcoplasmic reticulum calcium uptake of protein levels and the ability to Stachydrine reflect on NE-induced hypertrophy of neonatal rat cardiac myocytes studied. Result：Stachydrine can significantly reduce the protein content of myocardial cells and reduce Protein /DNA ratio;Stachydrine in a dose-dependent increase NE-induced cardiac mast cells in sarcoplasmic reticulum calcium uptake and activity of SERCA. Conclusion：Stachydrine on NE-induced cardiac mast cells in sarcoplasmic reticulum calcium uptake have a certain degree of increased dose-dependent relationship between certain,with the concentration of Stachydrine improvement of sarcoplasmic reticulum calcium uptake rate and activity of SERCA has improved markedly.

关 键 词：益母草水苏碱 心肌细胞 心肌肥大 钙摄取能力 SERCA活性 

分 类 号：R285.5[医药卫生—中药学]