高效液相色谱法测定止咳宁嗽胶囊中的绿原酸和橙皮苷  

Simultaneous Determination of Chlorogenic acid and Hesperidin in Zhike Ningsou Capsule by HPLC

作　　者：许海棠[1] 卢建芳[1] 蓝虹云[1] 

机构地区：[1]广西民族大学化学与生态工程学院,南宁530006

出　　处：《中国实验方剂学杂志》2010年第8期44-46,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西民族大学学校重点科研项目2009MDZD43

摘　　要：目的:建立高效液相色谱法同时测定止咳宁嗽胶囊中的有效成分绿原酸和橙皮苷的方法。方法:样品用甲醇超声提取,在ZorbaxSB-C18(3.0mm×250mm,5μm)色谱柱上分离,以含1%乙酸的水(A)-乙腈(B)体系为流动相进行阶梯洗脱,程序为0min(82∶18)～5min(82∶18)～5.1min(75∶25)～20min(75∶25);流速为0.35mL·min-1;327nm紫外检测。结果:绿原酸在进样量为0.4～10.0μg·mL-1内呈良好的线性关系;橙皮苷在进样量为6.2～155.0μg·mL-1内呈良好的线性关系。绿原酸的平均回收率为99.2%,RSD为1.0%。橙皮苷的平均回收率为99.6%,RSD为0.9%。结论:该方法简便快速,结果准确,重复性好,可为止咳宁嗽胶囊的质量控制提供参考依据。Objective：To establish a HPLC method for simultaneous determination of chlorogenic acid and hesperidin in Zhike Ningsou Capsule .Method：The sample was extracted with methanol under ultrasonication.The HPLC separation was performed on a Zorbax SB C18（3.0 mm × 250 mm,5 μm） column,using water containing 1% acetic acid（A）–acetonitrile（ B） as the mobile phase,in stepwise elution：0 min（82∶18）～5 min（82∶18） ～5.1 min（75∶ 25）～20 min（75∶25）,at a flow rate of 0.35 mL·min-1.Result：The linear range was 0.4～10.0 μg·mL-1 and 6.2～155.0 μg·mL-1 for chlorogenic acid and hesperidin; the average recovery was 99.2% and 99.6% ; RSD 1.0% and 0.9% ,respectively.Conclusion：This method can be applied for the quality control of Zhike Ningsou Capsule.

关 键 词：止咳宁嗽胶囊 绿原酸 橙皮苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]