HPLC双波长测定中药退黄外洗液中栀子苷、甘草苷、柚皮苷的含量  

HPLC determination of Geniposide,Liquiritin,Naringin of Zhongyaotuihuangwaixiye Lotion

作　　者：林冬杰 

机构地区：[1]梧州食品药品检验所,广西梧州543002

出　　处：《中国实验方剂学杂志》2010年第8期74-76,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究以HPLC法同时测定中药退黄外洗液中栀子苷、甘草苷、柚皮苷含量的方法。方法:采用高效液相色谱法。色谱柱Agilent SB-C18柱(4.6mm×250mm,5μm),流动相以甲醇-水(40∶60),流速1.0mL·min-1,检测波长,λ1238nm,λ2283nm,柱温30℃。结果:栀子苷在5.1～127.5ng线性关系良好(r=0.9997),平均回收率为99.41%,RSD0.24%(n=6);甘草苷在2.3～53ng线性关系良好(r=0.9995),平均回收率为98.49%,RSD1.23%(n=6);柚皮苷在4.9～123ng线性关系良好(r=0.9999),平均回收率为99.41%,RSD0.48%(n=6)。阴性对照无干扰。结论:该法操作简便,结果准确可靠,可作为中药退黄外洗液的质量控制方法。Objective：To establish the HPLC method for determination of geniposide liquiritin and naringin in Zhongyaotuihuangwaixiye Lotion.Method：A Agilent SB-C18 Column（4.6 mm×250 mm,5 μm） was used.The mobile phase was methanol-H2O.The column temperature was 30 ℃ .The waveleng detection was at 238 nm and 283 nm,,flow rate was 1.0 mL·min-1.Result：The linear range of Geniposide was within 5.1～127.5 ng（r=0.999 7）.The average recovery was 99.41% ,RSD was 0.24% （ n=6 ）.The linear range of Liquiritin was within2.3～53 ng （r=0.999 5）.The average recovery was 98.49% ,RSD was 1.23% （n=6）.The linear range of naringin was within4.9～123 ng （r=0.999 9）.The average revovery was 99.41% ,RSD was 0.48% （n=6）.The negative sample had no interference.Conclusion：This method is simple,accurate and suitable for its assaying.It can be used for quality control in production of Zhongyao Tuihuang Waixiye Lotion.

关 键 词：中药退黄外洗液 HPLC 栀子苷 甘草苷 柚皮苷 

分 类 号：R284.1[医药卫生—中药学]