莪术油对肺腺癌A549细胞周期及组织蛋白酶K表达的影响  

Influence of Zedoary Oil on Cell Cycle and Cathepsin K Expression in A549 Cell Line

作　　者：杨长福[1,2] 李亚东[1] 牛建昭[1] 王晓波[1] 黄春芳[1] 唐炳华[1] 赵丕文[1] 刘霞[1] 王继峰[1] 

机构地区：[1]北京中医药大学生化教研室,北京100029 [2]贵阳中医学院,贵阳550002

出　　处：《中国实验方剂学杂志》2010年第8期113-116,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部国际合作项目(2008DFA31970)

摘　　要：目的:探讨莪术油对A549细胞增殖抑制作用及组织蛋白酶K表达的影响。方法:MTT法观察莪术油对A549细胞增殖的抑制作用;流式细胞术检测细胞周期阻滞;Western blot检测组织蛋白酶K的表达情况。结果:莪术油作用后①MTT法结果显示莪术油在60mg·L-1～200mg·L-1对A549细胞增殖有抑制作用,IC50随作用时间的延长显著前移。②流式细胞术结果出现凋亡峰,与未处理组细胞相比,给药组细胞G0/G1期细胞比例增加,S期细胞比例降低。③Western blot结果显示,莪术油处理细胞24h后,组织蛋白酶K表达明显上调,100mg·L-1达最大值。结论:莪术油能抑制肺腺癌A549细胞增殖,阻滞细胞周期的作用,其机制可能与上调组织蛋白酶K表达水平有关。Objective：To explore the Zedoary oil on A549 cell line inhibiting the proliferation and cathepsin K expression.Method：The effects of Zedoary oil on the proliferation of lung adenocarcinoma cell line A549 were determined by MTT assay.The cells cyclic retardation were examined by using flowcytometry.Cathepsin K expression of level were detected by using western blot.Result：MTT showed that Zedoary oil had an inhibitory effect on the proliferation of A549 cells in 60～200 mg ·L-1.Flow cytometry showed that Zedoary oil increased the percentage of G0/G1 phase cells and reduced the percentage of S phase cells.The results of western blot showed that the expression of cathepsin K was up-regulated significangly in A549 cells of Zedoary oil groups compared with that in controls.Conclusion：Zedoary oil can significantly inhibit the growth of A549 cells,blocking the cell cycle.The effects may due to the up-regulation of cathepsin K.

关 键 词：莪术油 A549细胞 细胞周期 组织蛋白酶K 

分 类 号：R285.5[医药卫生—中药学]