长期超低温保存后真鲷精子的质量变化  

Quality change of Pagrus major sperm after long-term cryopreservation

作　　者：陈亚坤[1,2] 刘清华[1] 赵春彦[1] 肖志忠[1] 徐世宏[1] 马道远[1] 李军[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院研究生院,北京100039

出　　处：《海洋科学》2010年第6期50-54,共5页Marine Sciences

基　　金：中国科学院海洋研究所知识创新领域前沿项目(Y02507101Q)

摘　　要：通过对精子运动率、受精率和超微结构的观察，研究了2003-2009年分5批冷冻保存的真鲷（Pagrus major）精子经1～73个月保存后质量变化情况。冷冻精子在40℃水浴中解冻，大约100-110S转化为液态后取出，用海水激活后观察精子运动率，人工受精6～8h后观察受精率，同时精子用2．5％戊二醛前固定，经处理后用扫描电镜和透射电镜观察精子超微结构。结果表明，冷冻精子的最高运动率（87．67％±2．52％）和受精率（71．33％±8．84％）都是2009年保存1个月的精子，保存1，13，26个月的精子运动率和受精率差异不显著（P〈0．05），保存48个月后的精子运动率和受精率显著低于保存1个月的精子（P〈0．05），2003年保存的精子（73个月）运动率（50．67％±5.31％）和受精率最低（39．56％±0．69％）。精子的超微结构损伤主要包括精子头部损伤，线粒体损伤以及精子质膜的损伤。After cryopreservation （1-73 months）, the quality of Pagrus major sperm, which was cryopreserved from 2003 to 2009 in 5 batches, was examined by observing motility, fertilization rate and ultrastructures. The cryopreserved sperm were thawed in 40℃ water bath for 100-110 s. After activation with seawater, the motility of the sperm was estimated; 6-8 h after artificial fertilization, fertilization rate was examined; and after being fixed with by 2.5% glutaraldehyde, the ultrastructure of sperm was studied with scanning electron microscope and transmission electron microscope. The highest motility （87.67%±2.52%） and fertilization rate （71.33%± 8.84%） were obtained in sperm after a 1-month cryopreservation, even though they were not significantly different from those of the sperm after a 13-month or and 26-month cryopreservation （P〉0.05）. After a 48-month cryopreservation, the motility and fertilization rate decreased significantly in comparison with those of sperm after a 1-month cryopreservation （P 〈0.05）. The lowest motility （50.67% ± 5.31%） and fertilization rate （39.56% ±0.69%） were obtained in sperm cryopreserved for 73 months; main ultrastructural damage was observed in the head, mitochondria and membranes of sperm.

关 键 词：低温保存 真鲷(Pagrus major) 精子 运动率 受精率 

分 类 号：Q331[生物学—遗传学]