软骨素酶联合雪旺细胞移植促进脊髓损伤修复的研究  

The combined therapy of chondroitinase and Schwann cells transplantation after spinal cord injury in rats

作　　者：徐云强[1] 冯世庆[1] 王沛[1] 马信龙[1] 宁广智[1] 班德翔[1] 王希山[2] 

机构地区：[1]天津医科大学总医院骨科,300052 [2]山东省日照市人民医院骨科

出　　处：《中华实验外科杂志》2010年第7期972-975,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30872603）;天津市应用基础和前沿技术支持计划资助项目（07JCBJCl0200）;教育部新世纪人才支持计划资助项目（NCET-06-0251）

摘　　要：目的 观察软骨素酶联合雪旺细胞移植在治疗急性脊髓损伤中的作用.方法 Wistar大鼠80只,制作T10节段急性脊髓损伤模型（致伤力10g×4cm）.随机分成4组：对照组、雪旺细胞移植组、硫酸软骨素酶治疗组和联合治疗组.采用脊髓运动功能评分（BBB法,总分21）、神经电生理（SEP＆MEP）检查和生物素葡聚糖胺（BDA）神经示踪及标本NF-200免疫组织化学染色等比较各组疗效.结果 4周后BBB评分实验组较对照组明显提高,各组间差异有统计学意义（P〈0.05）（12周时4组分别为9.11±1.41、11.22±1.59、11.77±1.76和14.22±1.92）.术后4周起,神经电生理检查实验组动物较对照组差异有统计学意义（P〈0.05）,12周时4组MEP的波幅分别恢复至术前的28.8%、44.9%、49.0%和56.8%.BDA示踪显示联合组较对照组有较多的神经纤维穿过损伤部位.NF-200免疫组织化学染色吸光度（A）比较各组间差异有统计学意义（P〈0.05）,3个实验组纵切片A值与对照组的比值为1.44、1.55和2.78.结论 联合应用软骨素酶和雪旺细胞移植来治疗脊髓损伤,起到协同作用,效果好于单一方法,能明显促进脊髓损伤后的轴突再生和肢体功能恢复.Objective To investigate the effect of the combination of chondroitinase and Schwann cells （SCs） transplantation after spinal cord injury （SCI） in adult rats. Methods Eighty adult Wistar rats were divided into four groups the control group （A）, the Schwann cells transplantation group （ B）, the chondroitinase ABC（ChABC） group （C） and the SCs transplantation plus chondroitinase ABC group （D）. The thoracic T10 spinal cord injury model was made by Impactor model II manual （10g×4 cm）. After SCI, the rats in group B received SCs transplantation in injured sites, those in group C received ChABC treatment, and those in group D received the combined treatment. The rats in group A only received blank saline and medium. The locomotor functions were evaluated according to the BBB score scale （total 21）, and the neuroelectrophysiological changes （SEP＆MEP） were examined by electromyographic machine. Twelve weeks after SCI, biotinylated dextran amine （BDA） was injected into the cerebral cortex to trace the corticospinal tracts. Two weeks after the tracing, the animals were killed and the spinal cord frozen sections were made. The tissue sections were processed with Cy3 fluorescence stain, HE stain and anti NF-200 immuohistological stain. Results From 4 weeks after SCI, the rats in groups B, C and D had obviously higher BBB scores than in control group （P〈0.05） , and BBB scores in group D was the highest. The scores in groups A, B, C and D were 9. 11 ±1.41, 11.22 ±1.59, 11.77 ±1.76 and 14. 22 ± 1. 92 respectively 12 weeks later. In electromyographic examination （SEP＆MEP） , the rats in groups B, C and D gained better recovery than group A （P 〈0. 05）, and group D was the best. At the week 12, the MEP wave recovery rate was 28. 8% , 44. 9% , 49. 0% and 56. 8% in groups A, B, C and D respectively. In BDA tracing slides, some axons could cross the injured sites in group D. There was significant difference in anti-NF-200 stain area among four groups （P〈0.05）.

关 键 词：脊髓损伤 再生 雪旺细胞 软骨素酶 细胞移植 

分 类 号：R651.2[医药卫生—外科学]