首乌益智胶囊对血管性痴呆大鼠Notch/Delta信号通路基因表达的影响  

Effect of Shouwu Yizhi Capsule on Gene Expression in Notch/Delta Signal Pathway of Vascular Dementia Model Rats

作　　者：李长生[1] 杨晓妮[1] 张志友 王运满[3] 

机构地区：[1]山东省千佛山医院中医科,济南市经十路16766号250014 [2]泰安医学院预防医学系 [3]同济大学附属同济医院分院

出　　处：《中医杂志》2010年第7期642-644,650,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30672744)

摘　　要：目的探讨首乌益智胶囊治疗血管性痴呆的作用机制。方法用改良Pulsinelli四血管阻断法制造血管性痴呆大鼠模型42只,随机分为首乌益智胶囊组、脑复康组、模型组,每组14只,并设假手术组14只。造模后7天,首乌益智胶囊组灌胃给予首乌益智胶囊溶液(0.08g/ml),脑复康组灌胃给予脑复康溶液(0.02g/ml),模型组与假手术组均灌胃生理盐水。20天后利用实时荧光定量PCR检测Notch/Delta信号通路HES1、Mash1、β淀粉样前体蛋白(β-APP)的mRNA表达。结果与模型组、脑复康组比较,首乌益智胶囊组HES1基因表达明显增高(P<0.05),而Mash1和β-APP基因表达的下降无统计学意义(P>0.05)。结论首乌益智胶囊能有效激活Notch/Delta信号通路上HES1、Mash1、β-APP基因的相互作用,这可能是首乌益智胶囊治疗血管性痴呆的作用机制之一。Objective To investigate the effect and mechanism of Shouwu Yizhi Capsule （Capsule of Radix Polygoni Multiflori and Semen Coicis for dementia） on the Notch/Delta signal pathway in experimental rats with vascular dementia （VD）. Methods The models were established with the modified Pulsinelli 4 vessel occlusion method. They were randomized into Shouwu Yizhi Capsule group,piracetam group,model group,and sham operation group with 14 rats in each group. Seven days after modeling,the Shouwu Yizhi Capsule group was administered intragastrically Shouwu Yizhi Capsule solution 0.08g/ml,the piracetam group was prescribed intragastrically piracetam solution 0.02g/ml,and the model and sham operation groups were given intragastrically normal saline. Twenty days later,the mRNA expression of HES1,Mash1,and β-APP on Notch/Delta singal pathway was determined by real-time quantitative fluorescent PCR. Results Compared with the model and piracetam groups,the mRNA expression of HES1 the in Shouwu Yizhi Capsule group was significantly increased （P0.01）,while the decrease of mRNA expression of Mash1 and β-APP was not significant （P0.05）. Conclusion Shouwu Yizhi Capsule could effectively activate the interaction of HES1,Mash1,and β-APP on the Notch/Delta signal pathway,being the possible mechanism in the treatment of VD.

关 键 词：血管性痴呆 首乌益智胶囊 Notch/Delta信号通路 HES1 Mash1 β淀粉样前体蛋白(β-APP) 

分 类 号：R285.5[医药卫生—中药学]