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作 者:姜洪君[1] 郭凤根[1] 张丽梅[1] 陈严平[1] 李孙文[1] 杨芳[1]
机构地区:[1]云南农业大学农学与生物技术学院,云南昆明605201
出 处:《中国中药杂志》2010年第14期1812-1814,共3页China Journal of Chinese Materia Medica
基 金:云南省社会发展科技计划项目(2007C211M)
摘 要:目的:分析云南境内不同岩白菜居群的熊果苷含量,筛选出优良种源和最佳利用部位。方法:应用HPLC,色谱柱SB-C18,流动相甲醇-水(15∶85),流速1 mL.m in-1,检测波长282 nm,柱温30℃。结果:同一居群的不同部位间熊果苷含量差异悬殊,叶片>叶柄>根>根状茎;不同居群的叶片中的熊果苷含量存在极显著差异,介于6.36%~1.51%;不同居群的根状茎中的熊果苷含量也存在极显著的差异,在1.72%~0.40%。结论:叶片是提取熊果苷的最佳部位;兰坪老君山居群是开发利用岩白菜中熊果苷资源的最优种源。Objective: To determine arbutin contents in different populations of Bergenia purpurascens in Yunnan province,for screening out the best resource and best part of B.purpurascens.Method: The SB-C18 column was used with methanol-water(15∶85)as the mobile phase,at the flow rate of 1 mL·min-1 and column temperature of 30 ℃,and 282 nm was selected as the detected wavelength.Result: There were much significant differences in arbutin contents among various parts of the same B. population.The sequence of parts from high to low was laminapetiole root rhizome.Arbutin contents in laminae of different B.populations were different at the most significant level and varied between 6.36% and 1.51%.Arbutin contents in rhizomes of different B.populations were also dignificantly different at varied between 1.72% and 0.40%.Conclusion: Lamina is the best part for extracting arbutin.B.population distributed in Laojunshan mountain in Lanping county is the best resource for arbutin exploitation in B.purpurascens.
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