猫爪草多糖免疫调节及抗氧化活性研究  

Effects of polysaccharide of Radix Ranunculi Ternati on immunomodulation and anti-oxidation

作　　者：吕小华[1] 王会敏[2] 韩红霞[3] 吕世静[3] 覃冬云[1] 

机构地区：[1]广东医学院药理学教研室,广东湛江524023 [2]广东医学院临床免疫学教研室,广东湛江524023 [3]广东省中医院二沙岛分院检验科,广东广州510105

出　　处：《中国中药杂志》2010年第14期1862-1865,共4页China Journal of Chinese Materia Medica

基　　金：广东省自然科学基金项目(2007009940)

摘　　要：目的:探讨猫爪草多糖(polysaccharide of Radix Ranunculi Ternati,PRT)对小鼠免疫功能的调节及抗氧化活性研究。方法:用MTT法检测PRT对小鼠胸腺细胞、脾脏淋巴细胞和腹腔巨噬细胞增殖活性的影响;用中性红比色法检测PRT对小鼠腹腔巨噬细胞吞噬功能的影响;采用普鲁士兰法测定PRT的还原能力;水杨酸捕捉法测定PRT对.OH的清除能力;邻苯三酚自氧化法测定PRT对O2.-的清除能力。结果:25～400 mg.L-1PRT呈剂量依赖式增强小鼠胸腺细胞和脾脏淋巴细胞增殖功能以及巨噬细胞的吞噬功能;PRT在200 mg.L-1时,对巨噬细胞增殖作用最强;在不同浓度梯度,PRT均表现出一定的还原能力;PRT在8 g.L-1时,接近同浓度Vit C对.OH自由基的清除作用;PRT在800 mg.L-1时,对O2.-的清除率达到95.39%。结论:PRT能增强小鼠胸腺细胞、脾脏淋巴细胞和腹腔巨噬细胞增殖能力,且对巨噬细胞有一最佳作用浓度;PRT能增强巨噬细胞的吞噬功能,并呈剂量-效应关系;PRT具有一定的还原能力,具有较强的清除.OH和O.2-的能力。Objective： To study effects of polysaccharide of Radix Ranunculi Ternati（PRT） on immunological function and anti-oxidation activity of mouse.Method： Cell proliferations of splenocyte,thymocyte and peritoneal macrophage were measured by MTT colorimetry.The phagocytic function of peritoneal macrophage was measured by neutral red colorimetric method.The disoxidation power of PRT was measured by Prussian blue method.The clearing effect of PRT on hydroxyl radical was measured by salicylic acid capture method.The clearing effect of PRT on superoxide anion free radical was measured by pyrogallol auto oxidation method.Result： PRT among 25-400 mg·L-1 could enhance thymocytes and spleen lymphocyte proliferation and macrophage phagocytosis.PRT（200 mg·L-1） has the strongest macrophage proliferation.PRT in different concentration has shown some disoxidation effects.PRT in 8 g·L-1 has nearly the same ability of clearing ·OH by Vit C with the same concentration.The clearance rate of PRT on O-·2 is 95.39%.Conclusion： PRT can enhance the cell proliferation capability of thymocytes,spleen lymphocytes and peritoneal macrophages.PRT can enhance macrophage phagocytosis in a dose-response relationship.PRT has saome disoxidation power and strong ability of clearing·OH and O-·2.

关 键 词：猫爪草多糖 免疫功能 抗氧化 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]