银杏酮酯(GBE50)对心肌生理特性及细胞内游离钙的影响  

Effects of GBE50 on physiological characteristics and intracellular free calcium of myocardium

作　　者：王星禹[1] 张志雄[1] 刘爱华[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《中国中药杂志》2010年第14期1866-1870,共5页China Journal of Chinese Materia Medica

基　　金：上海市教委重点学科项目(J50301)

摘　　要：目的:观察银杏酮酯(GBE50)对心肌生理特性及细胞内游离钙的影响,探讨GBE50抗心律失常的机制。方法:采用常规离体器官实验法记录心房生理特性,激光共聚焦显微技术测定细胞内游离钙。结果:银杏酮酯各组(10,20,40,80,160 mg.L-1)对右心房收缩频率抑制率,除10 mg.L-1外,其他各组与对照组比较均具有显著性差异(P<0.05或P<0.01)。各组对右心房收缩力与对照组比较均具有显著性差异(P<0.05)。各组对左心房收缩力抑制率与对照组比较均具有显著性差异(P<0.05或P<0.01)。各组对左心房静息后增强效应与对照组比较均具有显著性差异(P<0.05或P<0.01)。40,80,160 mg.L-1的GBE50对左心房功能不应期呈延长效应(P<0.01);50 mg.L-1GBE50灌流后4,8,12 min心室肌细胞内[Ca2+]i比灌流前减少(P<0.01)。结论:银杏酮酯可降低右心房自律性,降低心房收缩力;延长左心房功能不应期(FRP),降低左心房的静息后增强效应的作用,且作用呈一定剂量依赖性。这些作用可能与减少心室肌细胞内游离钙浓度有关。Objective： To investigate effects of GBE50 on the physiological characteristics and intracellular free calcium of myocardium.Method： The preparations of isolated atria of guinea pigs were used for recording the myocardial physiological characteristics and the technique of Fluo-3/AM was used for measuring the concentration of free calcium in myocytes.Result： GBE50（10,20,40,80,160 mg·L-1）prominently inhibited the contractive force and decreased spontaneous beat of right atrium in concentration-dependent manner.GBE50（10,20,40,80,160 mg·L-1）inhibited the contractive force of left atrium,and markedly decreased the post rest potentiation of myocardial contractility in left atrium in concentration-dependent manner.GBE50（10,20,40,80,160 mg·L-1） prolonged the function refractory period of isolated guinea pig left atrium.50 mg·L-1 GBE50 decreased i of guinea pig ventricular myocytes in normal Tyrode＇s solution by（46.53±8.44）% in 12 minutes. Conclusion： GBE50 induces inhibitory effects of the spontaneous beat,contractility,post-rest potentiation and prolonged the function refractory period.GBE50 may decrease i of guinea pig ventricular myocytes.

关 键 词：银杏酮酯GBE50 心肌生理特性 细胞内游离钙 

分 类 号：R285.5[医药卫生—中药学]