白藜芦醇对TNF-α诱导类风湿关节炎成纤维样滑膜细胞的影响  

Effects of resveratrol on proliferation and apoptosis of TNF-α induced rheumatoid arthritis fibroblast-like synoviocytes

作　　者：田静[1] 高洁生[1] 陈进伟[1] 李芬[1] 谢希[1] 杜金烽[1] 王静[1] 毛妮[1] 

机构地区：[1]中南大学湘雅二医院,湖南长沙410011

出　　处：《中国中药杂志》2010年第14期1878-1882,共5页China Journal of Chinese Materia Medica

基　　金：2009湖南中医药科研计划项目(2009065)

摘　　要：目的:观察白藜芦醇(Res)对TNF-α诱导类风湿关节炎(RA)成纤维样滑膜细胞(FLS)增殖及凋亡的影响,探讨其对PI3K/Akt/BAD信号通路作用。方法:MTT比色法测定Res对TNF-α诱导RA FLS生长的抑制作用。流式细胞仪检测Res对RA FLS细胞周期及凋亡率的影响。Western-blot方法分析Res对TNF-α诱导RA FLS中磷酸化Akt蛋白及其下游效应分子磷酸化BAD蛋白表达的影响。结果:Res对TNF-α诱导RA FLS增殖具有抑制作用,呈时间剂量依赖性。随着Res浓度增加,RA FLS凋亡率增加,G0/G1期细胞增多,G2/M期及S期细胞减少,与TNF-α组比较,差异具有统计学意义(P<0.05)。Res可抑制TNF-α诱导RA FLS磷酸化Akt蛋白以及磷酸化BAD蛋白的表达。结论:Res通过阻滞RA FLS细胞周期、下调PI3K/Akt信号通路Akt磷酸化水平发挥抑制增殖、诱导凋亡作用,Res具有治疗RA的潜在价值。Objective： To observe the effect of resveratrol（Res） on in vitro proliferation and apoptosis of TNF-α induced rheumatoid arthritis fibroblast-like synoviocytes（RA FLS）,and further to investigate the PI3K/Akt/BAD signal mechanism.Method： The inhibition rate of RA FLS was examined by MTT assay.Cell cycle and the amount of apoptotic cells was measured by flow cytometry.PI3K/Akt/BAD signal transduction proteins expression was measured by western blot.Result： The living cells measured by MTT dose and time-dependently reduced in Res groups.In Res groups,the fraction of living cells in the S-phase and G2/M-phase decreased respectively,while that in G1-phase increased,the difference was statistically significant compared with the TNF-α group（P0.05）.Flow cytometry demonstrated that the apoptosis rate increased with increased Res concentration.Res inhibited TNF-α induced phosphorylation of Akt and BAD in RA FLS.Conclusion： Res can inhibit RA FLS proliferation and induce apoptosis through inhibition of PI3K/Akt/BAD signalling pathway.Res may provide a new therapeutic approach in treatment of RA.

关 键 词：白藜芦醇 滑膜细胞 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]