人参种源遗传关系的ISSR分析  

Genetic relationship analysis of ginseng by ISSR analysis

作　　者：许永华[1] 张爱华[1] 金慧[2] 方景焕 金永昌 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]吉林人参研究院,吉林通化134001 [3]韩国国家园艺科学院人参特作部

出　　处：《中草药》2010年第7期1164-1167,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30570187)

摘　　要：目的阐明不同种源人参的遗传关系。方法用ISSR分子标记研究来自人参主要分布区17个种源样本。结果从100个ISSR引物中筛选出10个条带清晰、多态性高的引物,共扩增出95条带,其中80条带为多态性位点,多态性条带比率(PPB)为84.21%,通过聚类分析,可将17个人参种源聚为4类。结论分子标记研究结果与人参的形态性状存在显著的相关性,较好地揭示了人参种源间的遗传关系,可为人参资源保育和良种选育提供科学依据。Objective To study the genetic polymorphism and genetic relationship of different resource of ginseng. Methods Genetic relationship in 17 accessions which came from different origins was measured by using polymerase chain reaction （PCR） with ISSR marker. Results Ten ISSR primers were selected from 50 ISSR primers and used for ISSR amplification. A total of 95 bands were generated, of which 80 bands were polymorphic bands （the percentage of polymorphic band, PPB=84.21%）. Seventeen germplasms of ginseng were clustered to four. Conclusion The similarity coefficients are calculated with NTsys 2.10e software and the dendrogram is constructed with UPGMA for providing a scientific basis on genetic breeding, differentiation, and new cultivar selection.

关 键 词：人参 种源 遗传关系 ISSR 

分 类 号：R284.2[医药卫生—中药学]