脉冲场凝胶电泳技术在本草基因组计划中的应用  

Application of Pulsed Field Gel Electrophoresis in the Herbal Genome Program (HerbGP)

作　　者：刘明珠[1] 白志川[1] 陈士林[2] 孙超[2] 何柳[2] 张进[2] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《世界科学技术-中医药现代化》2010年第3期447-451,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金面上项目(30900113):基于宏量ESTs的蛇足石杉转录组分析及石杉碱甲合成酶(HAS)基因的鉴定研究;负责人:罗红梅

摘　　要：本文着重介绍了脉冲场凝胶电泳(PFGE)在本草基因组测序中的应用,包括测定药用真菌染色体数目和基因组大小、构建基因组文库及绘制物理图谱,并对PFGE使用中一些待解决的问题做了简要介绍。Pulsed Field Gel Electrophoresis （PFGE）, which was developed in the 1980s, is mainly used to separate large linear DNA molecules （50 kb-12 Mb）. With the development and implementation of the herbal genome program （HerbGP）, PFGE is playing an increasingly critical role. In this study, we focus on the PFGE application in herbal genomic sequencing, including the measurement of the chromosome number and genome size of medicinal fungi, as well as construction of the genomic library and physical mapping. We also discuss the problems of how to use PFGE.

关 键 词：PFGE 本草基因组计划 基因组大小 基因组文库 物理图谱 

分 类 号：R378.3[医药卫生—病原生物学]