不同饲料饲养家蚕其肠道微生态优势菌群类型的组成及差异性  

Comparative analysis of the composition of dominant intestinal microflora in silkworm reared with different forages

作　　者：向芸庆[1] 王晓强[1] 冯伟[1] 周围[1] 谢洪霞[1] 万永继[1] 

机构地区：[1]西南大学生物技术学院,重庆北碚400716

出　　处：《生态学报》2010年第14期3875-3882,共8页Acta Ecologica Sinica

基　　金：国家现代农业产业技术体系建设专项资金

摘　　要：为探讨鳞翅目昆虫的生长发育及抗病性与肠道微生态状况的关系,以不同的桑科植物柘叶与桑叶分别饲养家蚕,采用纯培养分离检测技术、16S rDNA序列测定和系统发育分析方法,对4、5龄家蚕肠道优势菌群的类型进行了鉴定和差异性分析。结果表明:柘叶与桑叶饲养家蚕共有的优势菌群有短波单胞杆菌属(Brevundimonas)、寡养单胞菌属(Stenotrophomonas)、肠杆菌属(Enterobacter)和葡萄球菌属(Staphylococcus)4个类群。从桑叶饲养家蚕肠道中检索到的优势菌群还有气单胞菌属(Aeromonas)、短杆菌属(Brevibacterium)、柠檬酸杆菌属(Citrobacter)、埃希氏菌属(Escherichia)和克雷伯氏菌属(Klebsiella)5个类群,而从柘叶饲养家蚕肠道中检索到的优势菌群仅有假单胞菌属(Pseudomonas)和土壤杆菌属(Agrobacterium)2个类群。饲料的改变导致家蚕肠道微生态细菌种群组成的变化,从柘叶饲养家蚕肠道中分离出的优势菌群与桑叶饲养的家蚕相比,出现较大差异且不如桑叶饲养家蚕的菌群丰富。推测这种改变可能与柘叶饲养家蚕生长发育不良、容易患病具有相关性。The insect intestinal tract is a complex microecosystem that contains abundant microorganisms. To explore the relationship of the intestinal microflora of lepidopteran insects with host growth,development and disease resistance,we reared silkworm larvae with tricuspid cudrania leaves and mulberry leaves （both belong to the family Moraceae） respectively,used methods of pure culture isolation,16S rDNA sequence determination and phylogenetic analysis to investigate the gut microorganisms in fourth and fifth instar silkworm in order to find the composition changes of the microbial community that induced by forages. A total of 56 dominant strains were isolated from intestine of silkworm larvae and classified into twelve different genera according to the characteristics of colonial morphology,mycelial,physiological-biochemical identification and 16S rDNA phylogeny. These twelve genera are Brevundimonas,Stenotrophomonas,Enterobacter,Staphylococcus,Pseudomonas,Agrobacterium,Aeromonas,Brevibacterium,Citrobacter,Escherichia,Klebsiella and Bacillus. Only four genera of these dominant strains （Brevundimonas,Stenotrophomonas,Enterobacter and Staphylococcus） were common existence in the intestine of silkworm larvae reared with tricuspid cudrania and mulberry leaves. Besides,five genera of these dominant strains （Aeromonas,Brevibacterium,Citrobacter,Escherichia and Klebsiella） were detected in the intestine of the silkworm larvae which reared with mulberry leaves,while only two genera （Pseudomonas and Agrobacterium） were presented in the intestine of the silkworm larvae reared with tricuspid cudrania leaves. The composition of dominant intestinal microflora had shown significant difference between two kinds of silkworm larvae that reared with different forages. While the silkworm larvae were reared with tricuspid cudrania leaves,it was notable that the diversity of dominant intestinal microflora was considerably lower than that of silkworm larvae which reared with mulberry leaves. In addition,we made preliminary

关 键 词：微生态优势菌群 肠道 家蚕 类型 差异性 柘叶 桑叶 

分 类 号：S881.22[农业科学—特种经济动物饲养] Q938[农业科学—畜牧兽医]