明日叶叶的愈伤组织诱导和快速繁殖  

Callus induction and rapid propagation of leaves of Angelica keiskei Koidz

作　　者：郭治友[1] 罗应[1] 钱绍方[2] 

机构地区：[1]黔南民族师范学院生命科学系,贵州都匀558000 [2]昆明理工大学生物技术研究中心,云南昆明650224

出　　处：《广东农业科学》2010年第7期70-72,共3页Guangdong Agricultural Sciences

摘　　要：为解决生产上短时间快速获得大量的明日叶(Angelica keiskei Koidz)种苗,采用明日叶叶的不同部位作为外植体进行组织培养。结果表明,利于诱导愈伤组织的培养基是N6+6-BA2.0mg/L+NAA2.0～2.5mg/L;愈伤组织诱导丛生芽分化的最佳培养基是N6+6-BA2.0mg/L;丛生芽生根培养的最优培养基是N6+NAA0.5mg/L+AC3.0g/L,其中培养基中加入50.0mg/L的CH可延缓愈伤组织的衰老。The in vitro rapid progagation technique, callus induction of different part＇s leaves, multiplication of cluster buds and rooting induction of Angelica keiskei Koidz were tested to set up its asexual rapid propagation system for providing a great quantity of seedings. The results showed that the optimum medium for callus induction, cluster bud multiplication and rooting induction were N6＋6- BA2.0 mg/L＋NAA2.0-2.5 mg/L, N6＋6-BA2.0 mg/L and N6＋NAA0.5 mg/L＋AC3.0 g/L. Adding CH 50.0 mg/L could be anti-aging of callus in medium.

关 键 词：明日叶 叶 组织培养 愈伤组织 快速繁殖 

分 类 号：S636.9[农业科学—蔬菜学]