供体抗原特异性的CD4^+CD25^+ Treg对心脏移植小鼠体内B细胞功能的影响  

The influence of donor antigenic specificity regulatory T cells exert to B cell's function of rats after heart transplantation in mice

作　　者：肖亚[1] 黄赤兵[1] 张艮甫[1] 王平贤[1] 范明齐[1] 冯嘉瑜[1] 

机构地区：[1]第三军医大学新桥医院泌尿外二科,重庆400037

出　　处：《重庆医学》2010年第14期1789-1791,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(30571863)

摘　　要：目的观察供体抗原特异性的CD4+CD25+Treg对同种异系心脏移植小鼠受体B细胞功能的影响。方法以Balb/c小鼠为供体、C57bl/6小鼠为受体建立小鼠心脏移植模型,于术前1 d、术后2周分别将供体抗原特异性的CD4+CD25+Treg(1×106)于尾静脉注入受体体内,术后1个月抽取小鼠外周血,分离B细胞,检测其在抗Ig M抗体刺激下的增殖情况,检测小鼠外周血IgG、IgA水平,以判断应用CD4+CD25+Treg后小鼠体内B细胞功能状态。实验组:术前、术后2周使用CD4+CD25+Treg(n=8);阳性对照组:术前、术后未使用CD4+CD25+Treg(n=8);空白对照组:未移植小鼠(n=8)。结果实验证实,实验组B细胞增殖水平最低,空白对照组其次,阳性对照组最高,3组差异均有统计学意义(P<0.05)。外周血IgG、IgA水平检测证实,实验组与空白对照组比较差异无统计学意义(P>0.05),二者明显低于阳性对照组(P<0.01)。结论经尾静脉注入CD4+CD25+Treg后心脏移植受体体内B细胞的功能可明显受到抑制,CD4+CD25+Treg可通过对B细胞的这一作用调节受体免疫状态,从而达到控制排斥反应,诱导免疫耐受的作用。Objective To study the influence of donor antigenic specificity CD4＋CD25＋ regulatory T cells exert to B cell′s function of rats after heart transplantation in mice.Methods We first established heart transplantation model in rats,Balb/c rats as donor,C57bl/6 rats as recipient.The day before the operation and the day after two weeks of the operation,donor antigenic specificity CD4＋CD25＋ regulatory T cells（1×106） was injected to the recipient by caudal vein.After one month,we drew the peripheral blood of recipient rat,isolated B cells,and detected the proliferation level of B cells under the stimulation of IgM antibody.The level of IgG and IgA in the peripheral blood of recipient rat was detected too.So we could evaluate the functional status of B cells in vivo after heart transplantation.The experiment was divided into 3 groups：experimental group（n=8）,donor antigenic specificity CD4＋CD25＋ regulatory T cells was injected to the recipient before and after operation;positive control group（n=8）,donor antigenic specificity CD4＋CD25＋ regulatory T cells wasn′t injected to the recipient before and after operation;blank group（n=8）,normal rats without transplantation.Results The experiment proved that the proliferation level of B cells was lowest in experimental group,and highest in positive control group.There was significant deviation in three groups（P0.05）.The level of IgG and IgA in the peripheral blood wasn′t significant deviation between experimental group and blank group（P0.05）,while the level of these two group was significantly lower than the level of positive control group.Conclusion The functional of recipient′s B cells was suppressed when donor antigenic specificity CD4＋CD25＋ regulatory T cells was injected to the recipient by caudal vein,which shows that donor antigenic specificity CD4＋CD25＋ regulatory T cells can adjust the immune state of recipient by this way.That provides us a new way to suppress rejection and induce immune tolerance after heart tran

关 键 词：CD4+CD25+ TREG B细胞 抗体 小鼠 心脏移植 

分 类 号：R654.2[医药卫生—外科学]