造血干细胞向淋巴系祖细胞增殖分化过程中HOXC4基因表达的研究  

Research of the expression of HOXC4 gene in the differentiation and proliferation of the hematopoietic stem cell to lymphocytic progenitor cell

作　　者：邹艳[1] 刘文君[1] 翟雪松[1] 陈艾[1] 

机构地区：[1]泸州医学院附属医院儿科,四川泸州646000

出　　处：《重庆医学》2010年第14期1839-1841,共3页Chongqing medicine

基　　金：四川省教育厅重点基金资助项目(2004A058)

摘　　要：目的探讨人类脐血造血干细胞(HSC)向淋巴系祖细胞增殖分化过程中HOXC4基因表达的情况及全反式维甲酸(ATRA)对HOXC4基因表达的影响。方法 (1)采用造血干/祖细胞体外培养技术,以ATRA持续干扰人类造血干细胞,观察人类脐血HSC经植物血凝素(PHA-M)诱导后,在培养过程第3、7、12天的淋巴细胞集落形成单位(CFU-TL)生成情况。(2)采用实时荧光定量PCR技术检测HSC向淋巴系祖细胞增殖分化过程中HOXC4基因的表达水平。(3)用RNA表达相对量(2-△△Ct)表示HOXC4基因相对表达量,采用x±s表示HOXC4基因的变化情况。结果 (1)人脐血HSC向淋巴系祖细胞增殖分化过程中,HOXC4基因呈规律的表达。(2)随培养时间推移,HOXC4基因在增殖分化的第7天表达最强烈,第12天表达明显降低。(3)与正常组比较,ATRA可上调HOXC4基因的表达。结论 HOXC4基因在人脐血HSC向淋巴系祖细胞增殖分化过程中呈现规律表达,提示HOXC4基因与人类造血干细胞向淋巴系祖细胞增殖分化过程密切相关,是其调控基因之一。ATRA能显著上调HOXC4基因的表达。Objective To observe the expression of homeobox（HOX）C4 gene in the proliferation and differentiation of hematopoietic stem cell（HSC） to lymphocytic progenitor cell.And the differentiation progress was interfered by all-trans retinoic acid（ATRA） to observe the influence of ATRA on HOXC4 gene in this experiment.Methods（1）By the colony culture in vitro,the impact of ATRA on the hematopoietic stem-progenitor cell（HSPC） was surveyed,then observe the cell growth of colony forming unit-T lymphocyte（CFU-TL） in the differentiation progress of hematopoietic stem cell to lymphocytic progenitor cell which induced by PHA on the third,seventh,and twelfth day.（2） the expression of HOXC4 gene in the proliferation and differentiation of the HSC to lymphocyte progenitor cell in vitro was detected with real time fluorescence quantitive polymerize chain reaction（RT-FQ-PCR）.（3）The results were showed by means plus or subtracting standard deviation.Results（1）HOXC4 gene really has a regulatory function in the proliferation and differentiation progress of the HSC to lymphocytic progenitor cell.（2）Compared with the expression of HOXC4 gene on day 3,the quantity of HOXC4 gene was obviously higher on day 7 and lower on day 12 respectively in each group.（3）Compared with the expression of HOXC4 gene of normal group,the one of the ATRA group was up-regulated remarkably.Conclusion HOXC4 gene express regularly in the differentiation and proliferation of the hematopoietic stem cell to lymphocytic progenitor cell in human cord blood,which shows that there has a positive relationship between HOXC4 gene and the differentiation and proliferation of the hematopoietic stem cell to lymphocytic progenitor cell.ATRA can up-regulate the expression of HOXC4 gene.

关 键 词：造血干细胞 淋巴系祖细胞 HOXC4基因 全反式维甲酸 实时荧光定量PCR 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]