SSR Analysis of Three Species from Primary Parent and Their First Generation of Litopenaeus vannamei  

凡纳滨对虾3个亲本及其子代群体的SSR分析(摘要)(英文)

作　　者：栗志民[1] 谢丽[1] 叶富良[1] 陈国良 

机构地区：[1]广东海洋大学水产学院,广东湛江524025 [2]湛江海茂水产生物科技有限公司,广东湛江524000

出　　处：《Agricultural Science & Technology》2010年第3期57-61,共5页农业科学与技术（英文版）

基　　金：Supported by Technology Innovation Projects from Science and Tech-nology Department of Guangdong Province (2008A023700011)~~

摘　　要：Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique.The amplified products of genomic DNA with 8 microsatellite makers indicated that 8 loci presents polymorphism,the number of total alleles were 28 in 6 species;the number of allele of every loci was 2 to 6;mean allele number （Na） was 3.5;expected heterozygosity value （He） were higher than observed heterozygosity value （Ho）;mean polymorphism information content （PIC） ranged from 0.479 4 to 0.769 9,which showed these 8 loci posses relatively high information content.Through analysis of genetic structure in primary parent and their first filial,allele and effective allele number,observed heterozygosity and expected heterozygosity values and mean polymorphism information content （PIC） in filial generation were little lower than their primary parents.However,genetic diversity of filial generation is not influenced,and still maintains a good heritability.利用微卫星标记技术,采用8对微卫星引物对凡纳滨对虾3个亲本群体(OI Q、SIS Q、Kona Bay Q)及其子一代(OI Z、SIS Z、Kona BayZ)共计120个个体进行遗传分析。计算6个群体在8个微卫星位点上的平均等位基因数(Na)、平均有效等位基因数(Ne)、平均观测杂合度(Ho)、平均期望杂合度(He)、平均多态信息含量(PIC)和平均Hardy-Weinberg平衡指数(D),以上各参数均采用群体遗传学分析软件POP-GENE3.2处理,同时根据Nei的方法计算群体间的遗传距离和遗传相似系数。运用MEGA3.0软件,采取UPGMA方法根据6个群体的遗传距离进行聚类。结果表明:8对微卫星引物在6个群体中共检测到28个等位基因,每个位点的等位基因数为2 ～6个,平均等位基因数为3.5;各位点的期望杂合度均比观测杂合度高;多态信息含量(PIC)值为0.479 4 ～0.769 9,说明这8个位点在凡纳滨对虾中具有较高的信息含量。在亲本和子代群体遗传结构分析中,子代的有效等位基因数、杂合度和多态信息含量等指标均略低于亲本群体,但子代的遗传多样性并未受到太大影响,仍保持较好的遗传力。

关 键 词：Litopenaeus vanname MICROSATELLITE Brood and F1 stocks Genetic variation 

分 类 号：S917.4[农业科学—水产科学]