Effect of Metal Ions on Protease Activities in the Intestines and Hepatopancreas of Red-white Ornamental Carp (Cyprinus carpio L)  

金属离子对红白锦鲤肠及肝胰脏蛋白酶活性的影响(摘要)(英文)

作　　者：郭永军[1] 魏东[1] 白东清[1] 闫珊珊[1] 吴旋[1] 宁博[1] 陆建权[1] 

机构地区：[1]天津农学院水产科学系天津市水产生态及养殖重点实验室,天津300384

出　　处：《Agricultural Science & Technology》2010年第3期104-106,122,共4页农业科学与技术（英文版）

摘　　要：[Objective] The aim of this study was to study effects of metal ions on the protease activities in digestive tissues and gland of red-white ornamental carp（Cyprinus carpio L）.[Method] Effects of four kinds of metal ions （K＋,Na＋,Mg2＋ and Ca2＋） on protease activities in hepatopancreas,foregut,midgut,hindgut of red-white ornamental carp were studied by enzyme analysis method.[Result] Effects of four kinds of metal ions on protease activities of red-white ornamental carp were different in the range of experimental concentration from 25 mmol/L to 150 mmol/L.K＋ could promote protease activities in hepatopancreas and hindgut at different levels.Especially,K＋ had the promoting effect at low-concentration level,but the inhibitory effect at high-concentration level in midgut and the inhibitory effect in foregut.Na＋ had the promoting effect on protease activities in hepatopancreas,foregut and hindgut at different levels,but the inhibitory effect in midgut.Mg2＋ and Ca2＋ had the inhibitory effect on protease activities in intestinal and hepatopancreas at different levels.[Conclusion] This study provides basic data and theoretical foundation for researches on the digestive physiology of red-white ornamental carp or the development and optimization of compound feed.[目的]研究金属离子对红白锦鲤消化组织蛋白酶活性的影响。[方法]以酶学分析方法研究4种金属离子(Na+、K+、Ca2 +、Mg2 +分别来源于NaCl、KCl、CaCl2.2H2O、MgCl2.6H2O,浓度梯度均设置为25、50、75、100、125、150 mmol/L)对红白锦鲤肝胰脏、前肠、中肠、后肠蛋白酶活性的影响。蛋白酶活性的测定采用福林-酚显色法,用Thermo紫外分光光度计,根据OD680计算蛋白酶活性,以1 min水解酪蛋白产生1μg酪氨酸为一个酶活力单位,记为μg/(min.g)。[结果]在设定的浓度范围内(25 ～150 mmol/L),4种金属离子对蛋白酶活性影响不同。K+对肝胰脏和后肠的蛋白酶活性有不同程度的促进作用,其中K+对中肠表现为低浓度促进,高浓度抑制的作用,对前肠蛋白酶表现为抑制作用;Na+对肝胰脏、前肠和后肠的蛋白酶表现为不同程度促进作用,对中肠表现为抑制作用。Mg2 +、Ca2 +对肠及肝胰脏的蛋白酶均表现为不同程度的抑制作用。试验所选用的K+、Na+、Mg2 +、Ca2 +均属矿物元素,其中添加浓度25 mmol/LK+对锦鲤肠蛋白酶活性促进作用最大;浓度150 mmol/L Na+显著提高后肠和肝胰脏蛋白酶活性。这些浓度可作为饲料添加剂中的参考用量。Mg2 +、Ca2 +对锦鲤各消化器官(中肠除外)的抑制作用随浓度的增加蛋白酶活性降低,但考虑该元素系水产动物生长发育所必需,因此可选用低浓度或螯合物进行添加,以提高酶的生物学效价,提高饲料中蛋白的利用率,降低饵料系数,节约蛋白。[结论]该研究可为红白锦鲤消化生理的研究以及配合饲料的开发、优化提供基础数据和理论依据。

关 键 词：Red-white ornamental carp Metal ions Protease activities 

分 类 号：S917.4[农业科学—水产科学]