Morphological Changes of Hemocytes of Musca domestica Larva in vitro Infected by Escherichia coli  

家蝇幼虫离体血细胞感染大肠杆菌后的形态变化(摘要)(英文)

作　　者：晏容[1] 刘晖[1] 贺莉芳[1] 刘流[1] 

机构地区：[1]遵义医学院寄生虫学教研室,贵州遵义563003

出　　处：《Agricultural Science & Technology》2010年第3期115-117,共3页农业科学与技术（英文版）

基　　金：Supported by Health Department of Guizhou Province Foundation(D-281);Yong Foundation of Zunyi Medical College(F-241)~~

摘　　要：[Objective] The research aimed to observe the effects of Escherichia coli infection on the morphology of hemocytes of the 3rd stage larva of Musca domestica in vitro and understand the hemocytes types that take part in the cell immunity of Musca domestica larval.[Method] The hemcytes of the 3rd stage larva of Musca domestica were cultured in vitro and the hemocyte morphology was observed about 2,4,6,8 h after culture in vitro.After Escherichia coli were injected into the hemocytes of the 3rd stage larva of Musca domestica in vitro,the morphology changes of hemocytes were observed at different time after infection.[Result] The hemocytes of of the 3rd stage larva of Musca domestica was divided into five types about 2 h after hemoculture.The hemocytes partly adherence was seen about 6 h after hemoculture.The vacuolation and morpholysis was found in plasmatocytes after being infected by E.coli and a great quantity bacterium were gathered around granulocyte,but the morphology changes of hemocytes were not found in the prohemocyte,shprulocyte and oenocytoid.[Conclusion] The plasmatocyte and granulocyte were primary participants of the cell immunity of Musca domestica larval,but the prohemocyte,sphrulocyte and oenocytoid do not participate in the cell immune reactions.[目的]观察大肠杆菌感染对离体家蝇三龄幼虫血细胞形态的影响,了解参与家蝇幼虫细胞免疫反应的细胞类型。[方法]对家蝇三龄幼虫血细胞进行体外培养,并于培养后2、4、6、8 h对血细胞形态进行观察;离体家蝇三龄幼虫血细胞中加入大肠杆菌菌液,观察感染后不同时间血细胞形态的变化。[结果]家蝇三龄幼虫血细胞培养2 h后可区分血细胞的5种类型,即原血胞、浆血胞、粒血胞、珠血胞、类绛血胞,其中浆血胞又分为大核浆血胞和小核浆血胞2种;培养6 h细胞部分贴壁,少量浆血胞已生长成梭形,部分细胞伸出长短不等的伪足;培养8 h后培养血细胞发生黑化反应,液体发黑,不易观察;感染后2 h粒血胞周围见大量细菌聚集;感染后4 h浆血胞(主要是小核浆血胞)发生变形、碎裂、胞质普遍出现大小不等的空泡;感染后6 h浆血胞、粒血胞聚集成团将细菌包裹形成包囊;感染后8 h培养液完全黑化,已无法观察。,其中未见原血胞、珠血胞、类绛血胞形态的变化。[结论]浆血胞和粒血胞是家蝇幼虫细胞免疫反应的主要参与者,而原血胞、珠血胞及类绛血胞不参与细胞免疫反应。

关 键 词：Musca domestica larval Escherichia coli HEMOCYTE Morphology Cell immunity 

分 类 号：S852.4[农业科学—基础兽医学]