Extraction and Content Determination of Shikimic Acid in Pinus elliottii Engelm  

湿地松松针中莽草酸的提取及含量测定(摘要)(英文)

作　　者：谢济运[1,2] 陈小鹏[1] 李志荣[1] 陈芳[1] 粟本超[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004 [2]柳州师范高等专科学校,广西柳州545004

出　　处：《Agricultural Science & Technology》2010年第3期177-181,共5页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(30560119);Supported by Natural Science Foundation of Guangxi(0991030);Innovational Plan Project for Guangxi Postgraduate Ed-ucation(2008105930817M46);Project of Science Research Project of Guangxi Education Department([2006]26),Project of Science Research Project of Guangxi Education Department(200708LX180);Scientific Researc-hing fund project of Guangxi University(X081020);Fund Project for Guangxi Young People(0832008)~~

摘　　要：[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources.[Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the content of shikimic acid was determined by HPLC.The HPLC conditions were as follows;Alltima NH2 （5 μm,4.6 mm × 150 mm） column separation;the mobile phase was acetonitrile-2%H3PO4 （90：10）;the flowing velocity was 1 ml/min;test wavelength was 213 nm,the width of belt was 16 nm;reference wavelength was 300 nm,the width of belt was 80 nm.[Result] By the single factor and orthogonal tests,the optimum conditions were found as follows：ethanol concentration 60%,extraction temperature 75 ℃,solid-liquid ratio 1：25,extraction time 2.5 h.The extraction rate of shikimic acid was 1.49%.[Conclusion] Shikimic acid in Pinus elliottii Engelm could be used as a new resource to develop and utilize.[目的]研究湿地松针中莽草酸的提取工艺和含量,为更好地开发利用湿地松资源提供科学依据。[方法]用乙醇浸提湿地松松针中的莽草酸,研究不同水平乙醇浓度、料液比、浸提温度、提取时间及浸泡时间对莽草酸提取率的影响。并采用L9(34)正交试验,对固液比、提取时间、提取温度和乙醇的浓度各因素进一步进行正交试验研究,以寻找适宜的工艺条件。用高效液相色谱测定莽草酸含量。高效液相色谱的色谱条件:色谱柱:Alltima NH2(5μm,4.6 mm×150.0 mm);移动相:乙腈-2%H3PO4(90:10);流速:1ml/min;测定波长为213 nm,带宽为16 nm;参比波长为300 nm,带宽为80 nm。[结果]通过单因素和正交试验,确定影响莽草酸提取率的因素主次顺序是:乙醇浓度>提取温度>料液比>提取时间。乙醇浸提法提取莽草酸的最佳工艺:乙醇浓度为60%,提取温度为75℃,料液比为1:25,提取时间为2.5 h。在该条件下,莽草酸的提取率为1.49%。[结论]湿地松松针中的莽草酸可以作为新的莽草酸资源加以开发利用。

关 键 词：Pinus elliottii Engelm Shikimic acid Ethanol extraction Orthogonal test 

分 类 号：S791.22[农业科学—林木遗传育种]