镉与菲复合污染对安德爱胜蚓(Eisenia andrei)体腔细胞凋亡的影响  

Single and Joint Stress of Cd and Phe on Rate of Early Apoptotic Coelomcytes of Earthworms (Eisenia andrei)

作　　者：朱江[1,2] 

机构地区：[1]上海市环境科学研究院,上海200233 [2]上海交通大学环境科学与工程学院,上海200240

出　　处：《烟台大学学报（自然科学与工程版）》2010年第3期198-203,共6页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：上海市自然科学基金项目(09ZR1426700);上海市技术标准专项项目(10D20503900);国家基础研究重大项目(2004CB418503)

摘　　要：采用自然土壤染毒法,研究重金属镉(Cd)、多环芳烃菲(Phe)对安德爱胜蚓(Eisenia andrei)体腔细胞的毒性效应.利用流术细胞术检测Cd、Phe对细胞凋亡率和质膜损伤的影响.试验结果表明:体腔细胞的早期凋亡率是评估Cd-Phe复合污染毒性的敏感指标;Cd、Phe单一污染均表现出对蚯蚓体腔细胞质膜的损伤;对蚯蚓体腔细胞质膜损伤的大小顺序为Cd+Phe>Cd-Phe复合污染>单一的Cd或Phe;Cd-Phe复合污染对蚯蚓体腔细胞的毒性效为拮抗作用.流式细胞术能够灵敏、准确地表征Cd-Phe复合污染的毒性,是评价Cd-Phe复合污染程度的一种合理可靠的试验方法.Toxic effects of Cd（Cadmium） and Phe（Phenanthrene） on coelomocytes of earthworms（Eisenia andrei） is investigated by natural soil tests.Flow cytometry is used to examin the apoptosis rate of ceolomocytes caused by Cd and Phe pollution.The results show that both single and joint stress of Cd and Phe can cause early apoptosis of coelomocytes,the order of early apoptosis of coelomocytes is Cd＋PheCd combined Phesingle Cd or single Phe,and the joint effect of Cd and Phe is antagonistic.It suggests that FCM may be considered as a valuable and sensitive method to diagnose adverse effects of combined Cd and Phe.

关 键 词：镉 菲 复合污染 凋之 流式细胞术 

分 类 号：R99[医药卫生—毒理学]