细胞间黏附分子-1 K469E基因多态性与糖尿病视网膜病变的关系  

Relationship between Diabetic Retinopathy and K469E Gene Polymorphism of Intracellular Adhesion Molecule-1

作　　者：朱捷[1] 高适华[1] 池细俤[1] 张友胜[1] 赖小铮[1] 钟文亮[1] 

机构地区：[1]福建医科大学附属南平市第一医院眼科,南平353000

出　　处：《福建医科大学学报》2010年第3期190-193,共4页Journal of Fujian Medical University

基　　金：福建省卫生厅青年科研基金(2007-1-38)

摘　　要：目的探讨细胞间黏附分子-1(ICAM-1)469K/E基因多态性与糖尿病视网膜病变(DR)的关系。方法收集2型糖尿病无DR患者40例、2型糖尿病DR患者40例和对照者30例抗凝血。分离白细胞,以DNAzol抽提白细胞总DNA。分别采用基于BSt UI酶切位点的PCR-限制性长度多态性(PCR-RFLP)及等位基因序列特异性引物PCR法检测469K/E基因多态性。等位基因和基因型频率比较采用χ2检验。结果 PCR-RFLP及等位基因序列特异性引物PCR法检测469K/E基因多态性获得的结果完全吻合。2型糖尿病DR患者ICAM-1 469 KE及EE基因型出现的频率显著高于2型糖尿病无DR患者及对照组(P<0.01),2型糖尿病DR患者等位基因E出现的频率明显高于无DR患者及对照组(P<0.01)。结论 ICAM-1 469E等位基因与2型糖尿病视网膜病变有关。Objective To investigate the relationship between diabetic retinopathy and K469E gene polymorphism of intracellular adhesion molecule-1（ICAM-1）.Methods 40 cases of type 2 diabetes mellitus patients with and without retinopathy,30 cases of normal controls were enrolled.Anti-coagulated blood was collected,and genome DNA was extracted from isolated white blood cells by DNAzol reagents.The K469E gene polymorphism of ICAM-1 was detected by BStUI restriction site-based polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and allele sequence-specific primer PCR.Allele and genotype frequencies were analyzed by the χ2 test.Results Results from PCR-RFLP and allele sequence-specific primer PCR assay were consistent with each other.ICAM-1 469 KE and EE genotypes were significantly higher in type 2 diabetes mellitus patients with retinopathy than those without retinopathy and the control group（P0.01）,the frequency of alleles E in type 2 diabetes mellitus patients with retinopathy was apparent higher than that in patients without retinopathy and the control group（P0.01）.Conclusion K469E gene polymorphism of ICAM-1 is related with the diabetic retinopathy.

关 键 词：胞间黏附分子1 糖尿病视网膜病变 多态性 限制性片段长度 聚合酶链反应 

分 类 号：R587.1[医药卫生—内分泌]