Protective effects of transplanted neural stem cells on the brain of Alzheimer’s disease rats  

作　　者：Yan Zhan Dihui Ma Yu Zhang Ming Chang Linsen Hu 

机构地区：[1]Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin Province, China [2]Department of Neurology, First Hospital, Jilin University, Changchun 130021, Jilin Province, China

出　　处：《Neural Regeneration Research》2010年第11期825-832,共8页中国神经再生研究（英文版）

基　　金：the Jilin Pro-vincial Technology Devel-opment Foundation, No. 200505204, 200705129;the Postdoctorate Founda-tion from Northeast Normal University, No. 111258000

摘　　要：BACKGROUND： To date, no drugs are able to halt the progression of Alzheimer＇s disease （AD）. Neural stem cells （NSCs） transplantation has been widely used to treat AD, but the mechanism of AD treatment remains unclear. OBJECTIVE： To observe changes in protein and factors in the hippocampus and frontal lobe of AD rats following NSCs transplantation, and to understand mechanism of action of NSCs transplantation in AD treatment. DESIGN, TIME AND SETTING： A randomized, controlled animal study was conducted at the First Clinical Hospital, Jilin University, China from July 2007 to March 2009. MATERIALS： NSCs were harvested from the hippocampus of 10 E16 Wistar rats. METHODS： A total of 57 male adult Wistar rats were equally and randomly divided into normal control, AD model and NSCs groups. AD models were established in the AD model and NSCs groups by bilateral removal of hippocampus. At 2 weeks postsurgery, NSCs were transplanted into the hippocampus of rats from the NSCs group. MAIN OUTCOME MEASURES： Protein levels were measured in the hippocampus of rats from normal control, NSCs and AD model groups using proteomics. Expression of choline acetyl transferase mRNA, glial fibrillary acidic protein and S100β was measured in the hippocampus and frontal lobe of rats using in situ hybridization and immunohistochemistry. RESULTS： Expression of choline acetyl transferase mRNA, heat shock protein 70, heat shock protein 90, F-actin and actin was significantly higher in the NSCs group compared with AD model group. Glial fibrillary acidic protein and S100β expression was less in the NSCs group compared with AD model group. CONCLUSlOIN： NSCs implanted into the brain may generate new neural cells, which can relieve damage to the cholinergic system and resist apoptosis. NSCs transplantation plays a protective role in the cholinergic system in the AD rats to some extent.BACKGROUND： To date, no drugs are able to halt the progression of Alzheimer＇s disease （AD）. Neural stem cells （NSCs） transplantation has been widely used to treat AD, but the mechanism of AD treatment remains unclear. OBJECTIVE： To observe changes in protein and factors in the hippocampus and frontal lobe of AD rats following NSCs transplantation, and to understand mechanism of action of NSCs transplantation in AD treatment. DESIGN, TIME AND SETTING： A randomized, controlled animal study was conducted at the First Clinical Hospital, Jilin University, China from July 2007 to March 2009. MATERIALS： NSCs were harvested from the hippocampus of 10 E16 Wistar rats. METHODS： A total of 57 male adult Wistar rats were equally and randomly divided into normal control, AD model and NSCs groups. AD models were established in the AD model and NSCs groups by bilateral removal of hippocampus. At 2 weeks postsurgery, NSCs were transplanted into the hippocampus of rats from the NSCs group. MAIN OUTCOME MEASURES： Protein levels were measured in the hippocampus of rats from normal control, NSCs and AD model groups using proteomics. Expression of choline acetyl transferase mRNA, glial fibrillary acidic protein and S100β was measured in the hippocampus and frontal lobe of rats using in situ hybridization and immunohistochemistry. RESULTS： Expression of choline acetyl transferase mRNA, heat shock protein 70, heat shock protein 90, F-actin and actin was significantly higher in the NSCs group compared with AD model group. Glial fibrillary acidic protein and S100β expression was less in the NSCs group compared with AD model group. CONCLUSlOIN： NSCs implanted into the brain may generate new neural cells, which can relieve damage to the cholinergic system and resist apoptosis. NSCs transplantation plays a protective role in the cholinergic system in the AD rats to some extent.

关 键 词：neural stem cells Alzheimer＇s disease choline acetyl transferase glial fibrillary acidic protein S100Β proteomics 

分 类 号：Q813[生物学—生物工程] Q427