Feridex-labeled bone marrow stromal cells for analysis of sciatic nerve defects in rabbits  

作　　者：Guitao Li Xiaojun Tang Xiao He Dixin Luo Yong Qi Wangyang Xu 

机构地区：[1]Department of Orthopaedics, Guangdong Provincial Second People's HospitaI, Guangzhou 510317, Guangdong Province, China [2]Graduate School, University of South China, Hengyang 421001, Hunan Province, China [3]Department of Orthopaedics, Chenzhou First People's HospitaI, Chenzhou 423000, Hunan Province, China

出　　处：《Neural Regeneration Research》2010年第11期846-852,共7页中国神经再生研究（英文版）

基　　金：the Natural Science Foundation of Guangdong Province, No. 7301061

摘　　要：BACKGROUND： Traumatic approaches, such as sacrifice and perfusion sampling, have been used to evaluate efficiency of stem cell transplantation. However, these methods are not applicable to human studies. Cell tracing, in combination with non-invasive imaging technology, can be utilized to trace cell survival following transplantation to evaluate the efficacy of cell transplantation therapy. OBJECTIVE： To explore feasibility of magnetic resonance imaging （MRI） to observe in vivo repair of injured sciatic nerves following feridex and polylysine （FE-PLL） complex-labeled bone marrow stromal cell （BMSC） transplantation. DESIGN, TIME AND SE＇I-rlNG： A randomized, controlled, animal experiment was performed at the Laboratory of the Department of Neurosurgery, Zhujiang Hospital from March to December 2008. MATERIALS： Feridex was purchased from Advanced Magnetic, USA, and polylysine was purchased from Sigma, USA. METHODS： BMSCs were harvested from adult rabbit femurs and were cultured in vitro with neural stem cell culture medium, leukemia inhibitory factor, and basic fibroblast growth factor. Bone marrow stromal cell-derived neural stem cells （BMSC-D-NSCs） were obtained and labeled with FE-PLL complex. The right sciatic nerve （0.8 mm） was excised from healthy, New Zealand rabbits, aged 1.5 months, and the epineuria of distal stumps underwent turnover and were anastomosed at the proximal ends. FE-PLL labeled BMSC-D-NSC suspension or culture medium was transplanted into the epineunal lumen using a microsyringe. The left sciatic nerve was left intact and sewed as the normal control. MAIN OUTCOME MEASURES： Cellular morphology, proliferation, and differentiation, as well as expression of nestin and neuron-specific enolase （NSE）, of BMSCs-D-NSCs were observed. Efficacy of FE-PLL labeling and effects on cells were measured. In addition, neural regeneration at 2, 8, and 16 weeks following transplantation was observed by MRI. Histopathology and mean number of regenerated nerve fibers in the prBACKGROUND： Traumatic approaches, such as sacrifice and perfusion sampling, have been used to evaluate efficiency of stem cell transplantation. However, these methods are not applicable to human studies. Cell tracing, in combination with non-invasive imaging technology, can be utilized to trace cell survival following transplantation to evaluate the efficacy of cell transplantation therapy. OBJECTIVE： To explore feasibility of magnetic resonance imaging （MRI） to observe in vivo repair of injured sciatic nerves following feridex and polylysine （FE-PLL） complex-labeled bone marrow stromal cell （BMSC） transplantation. DESIGN, TIME AND SE＇I-rlNG： A randomized, controlled, animal experiment was performed at the Laboratory of the Department of Neurosurgery, Zhujiang Hospital from March to December 2008. MATERIALS： Feridex was purchased from Advanced Magnetic, USA, and polylysine was purchased from Sigma, USA. METHODS： BMSCs were harvested from adult rabbit femurs and were cultured in vitro with neural stem cell culture medium, leukemia inhibitory factor, and basic fibroblast growth factor. Bone marrow stromal cell-derived neural stem cells （BMSC-D-NSCs） were obtained and labeled with FE-PLL complex. The right sciatic nerve （0.8 mm） was excised from healthy, New Zealand rabbits, aged 1.5 months, and the epineuria of distal stumps underwent turnover and were anastomosed at the proximal ends. FE-PLL labeled BMSC-D-NSC suspension or culture medium was transplanted into the epineunal lumen using a microsyringe. The left sciatic nerve was left intact and sewed as the normal control. MAIN OUTCOME MEASURES： Cellular morphology, proliferation, and differentiation, as well as expression of nestin and neuron-specific enolase （NSE）, of BMSCs-D-NSCs were observed. Efficacy of FE-PLL labeling and effects on cells were measured. In addition, neural regeneration at 2, 8, and 16 weeks following transplantation was observed by MRI. Histopathology and mean number of regenerated nerve fibers in the pr

关 键 词：FERIDEX bone marrow stromal cells neural stem cells cell transplantation magnetic resonance imaging sciatic nerve RABBIT neural regeneration 

分 类 号：Q813.11[生物学—生物工程] TH776[机械工程—仪器科学与技术]