Synphilin-1 siRNA increases superoxide dismutase expression in a rat model of Parkinson’s disease  

作　　者：Anmu Xie Yongpeng Yu Xiang Xu 

机构地区：[1]Department of Neurology, Affiliated Hospital of Qingdao University Medical School, Qingdao 266003, Shandong Province, China

出　　处：《Neural Regeneration Research》2010年第12期885-889,共5页中国神经再生研究（英文版）

基　　金：the Young and Middle-Aged Scientists Research Foundation of Shandong Province, No. 200503021

摘　　要：BACKGROUND： Synphilin-1 has been shown to be involved in the pathogenesis of Parkinson＇s disease （PD）, and superoxide dismutase （SOD） reduction might induce PD onset. To date, the precise effect of synphilin-1 on SOD expression remains poorly understood. OBJECTIVE： To explore the influence of synphilin-1 small interfering RNA （siRNA） on SOD expression in a rat model of PD. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of Neurology, Affiliated Hospital of Qingdao University Medical School from June to October 2008. MATERIALS： 6-hydroxydopamine was purchased from Wuhan Boster, China. METHODS： A total of 40 male, Wistar rats were randomly assigned to PD, siRNA, siRNA negative control, and control groups, with 10 rats in each group. Rats from the PD, siRNA, and siRNA negative control groups were injected with 6-hydroxydopamine into the right substantia nigra to establish PD models. In addition, synphilin-1 siRNA and siRNA negative control sequences were separately injected into the right substantia nigra of siRNA, and siRNA negative control groups. The control group rats were treated with a mixture of ascorbic acid and normal saline. MAIN OUTCOME MEASURES： Synphilin-1 and SOD protein and mRNA expression in the substantia nigra was detected by immunohistochemistry and in situ hybridization. RESULTS： Synphilin-1 and SOD protein and mRNA expression were significantly decreased in PD and siRNA negative control groups （P 〈 0.05）. However, the siRNA group exhibited opposite effects. CONCLUSION： Synphilin-1 resulted in altered SOD expression, which suggested a protective role for synphilin-1 siRNA in an animal model of PD.BACKGROUND： Synphilin-1 has been shown to be involved in the pathogenesis of Parkinson＇s disease （PD）, and superoxide dismutase （SOD） reduction might induce PD onset. To date, the precise effect of synphilin-1 on SOD expression remains poorly understood. OBJECTIVE： To explore the influence of synphilin-1 small interfering RNA （siRNA） on SOD expression in a rat model of PD. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of Neurology, Affiliated Hospital of Qingdao University Medical School from June to October 2008. MATERIALS： 6-hydroxydopamine was purchased from Wuhan Boster, China. METHODS： A total of 40 male, Wistar rats were randomly assigned to PD, siRNA, siRNA negative control, and control groups, with 10 rats in each group. Rats from the PD, siRNA, and siRNA negative control groups were injected with 6-hydroxydopamine into the right substantia nigra to establish PD models. In addition, synphilin-1 siRNA and siRNA negative control sequences were separately injected into the right substantia nigra of siRNA, and siRNA negative control groups. The control group rats were treated with a mixture of ascorbic acid and normal saline. MAIN OUTCOME MEASURES： Synphilin-1 and SOD protein and mRNA expression in the substantia nigra was detected by immunohistochemistry and in situ hybridization. RESULTS： Synphilin-1 and SOD protein and mRNA expression were significantly decreased in PD and siRNA negative control groups （P 〈 0.05）. However, the siRNA group exhibited opposite effects. CONCLUSION： Synphilin-1 resulted in altered SOD expression, which suggested a protective role for synphilin-1 siRNA in an animal model of PD.

关 键 词：synphilin-1 siRNA superoxide dismutase rats Parkinson＇s disease degenerative nerve disease neural regeneration 

分 类 号：Q554.9[生物学—生物化学] Q522