遗传性乳光牙与正常恒牙牙本质表面形貌及元素分布和显微硬度的差异  

Differences of surface morphology,element content and micro-hardness between dentinogenesis imperfecta and normal permanent dentin

作　　者：席戈[1] 张东[1] 孟永洁[2] 王建荣[1] 刘斌[1] 

机构地区：[1]兰州大学口腔医学院修复科,甘肃省兰州市730000 [2]甘肃省人民医院质控科,甘肃省兰州市730000

出　　处：《中国组织工程研究与临床康复》2010年第24期4458-4461,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：兰州市科技局科技计划项目(2008-1-86;口腔多功能义齿的研制及临床应用研究);中国科学院兰州化学物理研究所固体润滑国家重点实验室开放项目(0808;口腔抗菌生物材料的制备及摩擦学研究);教育部大学生创新性实验计划项目(LZUYX200625;抗菌义齿的临床应用研究)~~

摘　　要：背景:目前国内外对遗传性乳光牙本质的研究主要集中于牙本质发育异常家系调查和表型分析,对遗传性乳光牙本质的物理性能的研究鲜有报道。目的:对比研究遗传性乳光牙与正常恒牙的牙本质的表面形貌,元素分布以及显微硬度的差异。方法:收集3例遗传性乳光牙本质患者需要拔除的牙齿,共5颗。选择年龄、性别相匹配的无龋坏的正畸牙作为对照的正常恒牙。采用扫描电镜,X射线能量色谱仪,纳米压痕仪对遗传性乳光牙和正常恒牙的牙本质进行表面形貌观察,元素分布以及显微硬度进行测试。结果与结论:遗传性乳光牙的牙本质结构松散,牙本质小管结构改变;遗传性乳光牙的牙本质中钠、锌、硫、钙含量显著高于正常恒牙,而镁、磷、氯降低正常恒牙(P<0.01);遗传性乳光牙的牙本质中钙/磷比值为1.76,明显高于正常恒牙(P<0.01)。遗传性乳光牙的牙本质的显微硬度是(1.18±0.18)GPa,明显低于正常恒牙牙本质的显微硬度(1.72±0.48)GPa(P<0.01)。因此,说明遗传性乳光牙本质小管结构发生改变,其硬度低于正常恒牙牙本质的硬度,这可能与遗传性乳光牙本质元素分布有关。BACKGROUND：At present,the study of dentinogenesis imperfecta focuses on familial and phenotypic analysis,but the reports about physical properties of dentinogenesis imperfecta dentin are few.OBJECTIVE：To comparative the differences of surface morphology,element content and micro-hardness of dentinogenesis imperfecta and permanent dentin.METHODS：Totally 5 dentinogenesis imperfecta teeth were collected from 3 patients.At the same time,orthodontic teeth served as controls.The surface morphology,element content and micro-hardness of dentinogenesis imperfecta dentin and permanent dentin were measured by scanning electron microscope,X-ray energy chromatography and nano-indentation instrument.RESULTS AND CONCLUSION：The dentin structure of dentinogenesis imperfecta was loose,and the dentinal tubule structural was affected.The content of Na,Zn,S,Ca in dentinogenesis imperfecta was significantly greater than that of permanent dentin,but the content of Mg,P,Cl was significantly decreased than permanent dentin（P 0.01）.The Ca/P ratio of dentinogenesis imperfecta was 1.76,which significantly higher than permanent dentin（P 0.01）.Microhardness test results showed micro-hardness of dentinogenesis imperfect was（1.18±0.18） GPa,which was significantly lower than that of normal permanent dentin（1.72±0.48） GPa（P 0.01）.The dentinal tubule structural of dentinogenesis imperfecta was changed,and micro-hardness was reduced,all these may be related to the element content.

关 键 词：遗传性乳光牙本质 元素含量 显微硬度 硬组织构建 口腔组织工程 

分 类 号：R314[医药卫生—基础医学]