两种主动免疫特发性血小板减少性紫癜动物模型免疫功能的动态变化  

Dynamic changes in immune function of active immunization-induced idiopathic thrombocytopenic purpura animal models

作　　者：胡海燕[1] 李章球[1] 余力[1] 姚瑶[1] 王国征[1] 孙明[1] 黄睿[1] 张梅霞[1] 陆志刚[1] 

机构地区：[1]南方医科大学附属珠江医院血液科,广东省广州市510282

出　　处：《中国组织工程研究与临床康复》2010年第24期4462-4468,共7页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省自然科学基金"树突细胞疫苗治疗特发性血小板减少性紫癜的实验研究"(07005195);广东省医学科学技术研究基金(WSTJJ20061116440105196211238024)~~

摘　　要：背景:目前的特发性血小板减少性紫癜模型多为重度,血小板下降显著但持续时间短,与临床患者血小板波动变化的特性拟合不佳。目的:用兔抗小鼠血小板血清及豚鼠抗小鼠血小板血清构建中度特发性血小板减少性紫癜模型,并探讨其适用性。方法:应用不同稀释倍数的抗血小板血清腹腔注射免疫小鼠,筛选出1:32稀释倍数的抗血清制备中度特发性血小板减少性紫癜模型。免疫结束后分8个时间点取血、骨髓及淋巴器官,观察各种细胞、细胞因子及组织结构的改变。结果与结论:两种抗血清免疫后,小鼠血小板数量均下降到正常数目的40%～60%,并能维持7d;骨髓巨核细胞数于免疫后8d明显增加,16d基本恢复正常;红细胞C3b受体花环与红细胞免疫复合物花环率明显下降(P<0.05);白细胞介素2和干扰素γ水平及CD4+/CD8+细胞比例明显升高(P<0.05);脾小巨核细胞增加,胸腺萎缩。说明豚鼠和兔抗小鼠血小板血清制备的慢性特发性血小板减少性紫癜模型存在红细胞免疫功能紊乱,T淋巴细胞亚群比例失调以及细胞因子分泌变化,是一种与临床拟合良好的中度特发性血小板减少性紫癜模型。BACKGROUND：Currently,most of the idiopathic thrombocytopenic purpura（ITP） models are severe and characterized by notable decrease of platelet and short persistence time,which can not fit to clinical platelet fluctuation features.OBJECTIVE：To construct mild ITP models induced by injecting anti platelet serum（APS） from guinea pig and rabbit,moreover,to explore the adaptivity of two methods METHODS：Mice were divided into guinea pig anti-mouse APS group and rabbit anti-mouse APS group in which mice were injected with APS of 1：32 dilution to establish mild ITP models.At 8 different sampling times after infusion,blood,bone marrow and lymphatic organ were collected to observe the changes of cells,cytokines and tissue structures.RESULTS AND CONCLUSION：Counts of platelet in both groups were decreased to 40%-60% of the normal number and maintained for 7 days.Counts of megakaryocyte were increased significantly at 8 days and gradually returned to normal at 16 days.Rates of C3bRR,ICR in both APS groups were decreased significantly compared with saline group（P 0.05）.The levels of interleukin 2 and interferon γ in both APS groups were elevated compared with saline group（P 0.05）.For pathology change,there was more micromegakaryocyte in spleen and thymus gland analosis significantly.There was imbalance of T lymphocytes subpopulations,disorders of immuno-functions of red blood cell and cytokine changes in two successful mild ITP models.The constructed model is fit for the clinical features of moderate ITP.

关 键 词：血小板减少性紫癜 动物模型 抗血小板血清 血小板 免疫功能 

分 类 号：R318[医药卫生—生物医学工程]