原位杂交技术在组织工程研究中的应用  

In situ hybridization in tissue engineering

作　　者：彭琴[1] 王远亮[1] 李玉筱[1] 

机构地区：[1]重庆大学生物工程学院,国家"985工程"生物材料与仿生工程研究中心,重庆市400044

出　　处：《中国组织工程研究与临床康复》2010年第24期4499-4502,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(30870609;30600130)资助~~

摘　　要：背景:通过原位杂交技术对修复组织进行检测,是目前分析组织再生和修复状况的重要方法之一。目的:综述国内外关于原位杂交技术在组织工程应用中的研究概况。方法:应用计算机检索PubMed1980-01/2009-07期间的相关文章,检索词为"In situ hybridization,Tissue engineering,Insulin-like growth factor-I(IGF-I)",并限定文章语言种类为English。同时计算机检索中国期刊全文数据库1995-01/2009-07期间的相关文章,检索词为"原位杂交,组织工程,胰岛素样生长因子1",并限定文章语言种类为中文。此外还手工查阅相关专著数部。纳入原位杂交技术基本原理及原位杂交技术在组织工程中相关应用的文章。结果与结论:原位杂交技术包括分子生物学、细胞遗传学和组织化学等方面的技术,是一种核酸定位和检测基因表达的有效方法。原位杂交技术尤其是荧光原位杂交技术,可对修复的组织进行准确有效的检测,在骨、软骨等组织工程实验研究中有广泛应用,已成为目前分析组织再生和修复状况的重要方法之一。近年来国内外对原位杂交技术在组织工程中的应用研究主要从生物医学和临床医学两方面着手,都获得了众多研究成果。此外,对与组织生长发育密切相关的生长因子胰岛素样生长因子1的原位检测也很有效,三维支架中胰岛素样生长因子1的原位检测是今后工作的重点。BACKGROUND：In situ hybridization for detecting repaired tissue is an important tool to analyze tissue regeneration and repairing status.OBJECTIVE：To summarize the in situ hybridization in tissue engineering.METHODS：A computer-based online search was conducted in Pubmed for English language publications using the key words of ＂In situ hybridization,Tissue engineering,Insulin-like growth factor-I（IGF-I）＂ from January 1980 to July 2009.Relevant data were also searched in Chinese Journal Full-text Database with the same key words in Chinese from January 1995 to July 2009.Articles about mechanism and application of in situ hybridization were included. RESULTS AND CONCLUSION：In situ hybridization was an important instrument for the study of nucleic acid localization and the detection of gene expression,and it included molecular biology,cytogenetics,histochemistry and so on.In situ hybridization,in particular fluorescence in situ hybridization,might accurately and effectively detect the repaired tissue.The applications of in situ hybridization in tissue engineering have been achieved greatly,such as bone and cartilage.Many results present a good promise in biomedical and clinical medical fields in recent years.Besides,it is effective to in situ detection of insulin-like growth factor-I（IGF-I）,which is closely related to growth and development of tissue.The emphasis of the future studies is about in situ detection of IGF-I in three-dimensional scaffold.

关 键 词：原位杂交 组织工程 胰岛素样生长因子1 组织再生 修复 

分 类 号：R318[医药卫生—生物医学工程]