复合缓释载体提高脂肪移植成活率的研究  

Study on Effect of PluronicF-127 and Vascular Endothelial Growth Factor Composite Delivery System for Improving the Survival of Grafted Fat

作　　者：徐靖宏[1] 刘友山[1] 谈伟强[1] 洪晓娅[1] 

机构地区：[1]浙江大学医学院附属第一医院整形外科,杭州310003

出　　处：《生物医学工程学杂志》2010年第3期600-605,共6页Journal of Biomedical Engineering

基　　金：浙江省卫生科研基金资助项目(2006B047)

摘　　要：探讨以PluronicF-127为血管内皮细胞生长因子(VEGF)载体构建的复合物对脂肪组织血管生长及移植成活率的影响。从人体获取脂肪,经纯化,消化后按加入物不同分成4组,即实验组(脂肪+30%PluronicF-127+20ng/mlVEGF)、VEGF对照组(脂肪+20ng/mlVEGF)、PluronicF-127对照组(脂肪+30%PluronicF-127)、空白对照组(单纯脂肪)。各组混合物分别注射于裸鼠背部皮下。在3、6和12周分别取材,行移植物质量、电镜超微结构、组织学染色和微血管CD34染色检测。结果显示,实验组的移植物重量和血管密度均高于对照组,具有统计学意义,其余各对照组间差别无统计学意义。提示PluronicF-127为载体构建的VEGF复合物能促进移植后脂肪组织血管的生成从而提高脂肪移植的成活率。To explore the effect of PluronicF-127 and Vascular endthelial growth factor（VEGF） delivery system on the survival of the grafted fat,we divided fat harvest under the same condition into 4 groups.One group served as blank control; the other 3 groups served for experiments with respective to DMEM containing 20 ng/ml VEGF; DMEM containing 30% Pluronic F-127;DMEM containing 20 ng/ml VEGF and 30% Pluronic F-127,and then we transplanted the 4 groups of fat,subcutaneously,on the back of 3 groups of BALB/c nude mice（8 mice per group; injecting 4 points per mouse;0.2 ml per point）. At 3 weeks,6 weeks and 12 weeks,we dissected the fat grafts,measured their weight retention,and put them in histopathologic examination with the use of HE and CD34 staining. And we compared the weight retention and microvessel density （MVD） of each experiment group versus those of control group. The relation between adipose cell and PluronicF-127 was observed through electron microscope.The results reveal that the MVD and weight of pluronicF-127 and VEGF of the experiment groups are significantly greater than those of other groups. The PluronicF-127 and VEGF composite delivery system can significantly improve the blood circulation for fat transplantation,and increase the survival rate of grafted fat.

关 键 词：脂肪移植 PluronicF-127 血管内皮细胞生长因子 微血管密度 

分 类 号：R318.0[医药卫生—生物医学工程]