巨噬细胞炎症蛋白-2抗体对肺型氧中毒小鼠肺组织的保护作用  

Protective effect of macrophage inflammatory protein-2 antibody in lung tissue of pulmonary oxygen toxicity in mice

作　　者：王世锋[1] 朱嘉[1] 吕传禄[1] 王洋[2] 方以群[1] 王文波[1] 

机构地区：[1]海军医学研究所,上海200433 [2]第二军医大学附属长海医院病理科

出　　处：《中华航海医学与高气压医学杂志》2010年第3期136-139,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的 观察吸入巨噬细胞炎症蛋白-2（macrophage inflammatory protein-2,MIP-2）拮抗剂对肺型氧中毒小鼠肺组织的保护作用.方法 40只健康C57 BL/6雄性小鼠随机数字法分为5组：对照组、0.2 MPa-224 min组,0.2 MPa-289 min组、高压常氧组、抗体组.小鼠在密闭氧舱中吸入高压纯氧建立肺型氧中毒模型;对照组吸入空气;高压常氧组吸入氮氧混合气,氧分压与空气中氧分压相同;抗体组雾化吸入MIP-2抗体气溶胶.动物出舱后0.5 h解剖取肺,肺组织用于病理检查和免疫组化方法检测MIP-2的含量.结果 高压氧暴露组与对照组相比,肺组织MIP-2含量明显增高（P〈0.05）;高压常氧组与对照组相比,肺组织MIP-2含量变化不明显（P〉0.05）;吸入MIP-2抗体后的抗体组肺组织损伤程度明显减轻.结论 随着高压氧暴露时间的延长,炎症损伤呈加重趋势,吸入MIP-2抗体可以在一定程度上减轻肺组织的炎症损伤.Objective To observe the effects of MIP-2 antibody on the inflammation in lung tissue of pulmonary oxygen toxicity in mice.Methods The pulmonary oxygen toxicity model were duplicated by breathing hyperbaric oxygen,and 40 mice were randomly divided into 5 groups：control group.0.2 MPa-224 min group,0.2 MPa-289 min group,normal O2 group,and antibody group.Mice in the control group breathed normal air,the normal O2 group breathed N2-O2 gas mixture,in which O2 partial pressure was same to that in the atmosphere.The antibody group breathed MIP-2 antibody aerosol.The expression of MIP-2 in the lung was measured by IHC.Results The MIP-2 abundance of HBO treated groups was significantly higher than that of the control group （P〈0.05）,whereas the expression of MIP-2 of the normal O2 group was not significantly higher than that of the control group （P〉0.05）.MIP-2 antibody may alleviate inflammation lesion of pulmonary oxygen toxicity in mice.Conclusions Pulmonary oxygen toxicity might elevate expression of MIP-2,and MIP-2 antibody alleviate inflammation lesion of pulmonary oxygen toxicity in mice.

关 键 词：高压氧 肺型氧中毒 巨噬细胞炎症蛋白-2抗体 

分 类 号：R686[医药卫生—骨科学]