自发性高血压大鼠心脏重构与ACE2 mRNA的表达  

Relationship between cardiac remodeling and expression of angiotensin-converting enzyme 2 in spontaneously hypertensive rats

作　　者：吴逸南[1] 贺红[2] 姜虹[2] 葛志明[2] 李方[2] 张运[2] 

机构地区：[1]山东中医药大学第二附属医院心内科,山东济南250001 [2]山东大学齐鲁医院心内科,山东济南250012

出　　处：《心脏杂志》2010年第4期517-519,共3页Chinese Heart Journal

摘　　要：目的:观察不同月龄的自发性高血压大鼠(SHR)的心脏血管紧张素转换酶2(ACE2)mRNA表达水平,探讨心脏重构与ACE2的内在联系。方法:将12周龄雄性SHR 18只和12周龄WKY Wistar-Kyoto rats大鼠18只随机分为两组,从WKY大鼠组和SHR组中各抽取9只处死,剩余的9只再喂养12周后处死。测量大鼠心脏的质量(HW)与体质量(BW)并计算HW/BW的比值。以实时定量RT-PCR法检测ACE2 mRNA的表达。结果:①与同周龄WKY大鼠组比较,SHR组HW/BW的比值显著增加(P<0.01);与12周龄SHR组比较,24周龄SHR组的HW/BW显著增加(P<0.05)。②与同周龄的WKY大鼠组比较,SHR组ACE2 mRNA的表达显著降低(P<0.01);与12周龄的SHR组比较,24周龄的SHR组ACE2 mRNA的表达显著降低(P<0.01)。结论:自发性高血压大鼠心脏重构伴随着心脏中ACE2 mRNA的表达下调。AIM： To investigate the relationship of cardiac remodeling and expression of angiotensin-converting enzyme （ACE） 2 mRNA in spontaneously hypertensive rats （SHRs）. METHODS： Eighteen male （12 weeks of age） SHRs and 18 male （12 weeks of age） Wistar Kyoto （WKY） rats were randomly divided into two groups： WKY control group （n=18） and SHR group （n=18）. Nine rats were randomly selected from each group and sacrificed immediately, whereas the other nine rats in each group were sacrificed 12 weeks later. Heart weight/body weight （HW/BW） was measured and mRNA expression of ACE2 was determined by real-time quantitative RT-PCR in all rats. RESULTS： HW/BW in 12- and 24-week-old SHRs increased compared with WKY rats （P〈0.01, P〈0.01）. HW/BW in 24-week-old SHR rats increased compared with 12-week-old SHRs （P〈0.05）. Myocardial expression of ACE2 mRNA in 12- and 24-week-old SHR rats was significantly lower than in WKY rats （P〈0.01, P〈0.01）. Myocardial expression of ACE2 mRNA in 24-week-old SHRs was significantly lower than in 12-week-old SHRs （P〈0.01）. CONCLUSION： Cardiac remodeling is related to expressions of ACE2 mRNA in SHRs.

关 键 词：血管紧张素转换酶2 自发性高血压 心脏重构 大鼠 

分 类 号：R544.1[医药卫生—心血管疾病]