HBV感染者外周血CD4^+CD25^+调节性T细胞的表达水平及临床意义  

Expression and clinical significance of CD4^+ CD25^+Treg in patients with chronic hepatitis B virus infection

作　　者：李浩[1] 徐洪涛[2] 邢同京[2] 咸建春[2] 叶军[2] 张立新[2] 

机构地区：[1]南通大学医学院,江苏226001 [2]南通大学附属泰州市人民医院南院肝病科

出　　处：《肝脏》2010年第3期174-176,共3页Chinese Hepatology

摘　　要：目的比较CD4+CD25+FoxP3+和CD4+CD25+CD127-/low两种设门方法对CD4+CD25+调节性T细胞(Treg)细胞的界定效果,并探讨HBV感染者外周血中Treg水平的表达及其临床意义。方法选择慢性乙型肝炎患者52例,HBV携带者24例,非活动性HBsAg携带者24例,正常对照者25例,采用CD4+CD25+CD127-/low设门方法用流式细胞仪进行检测,其中正常对照组和21例慢性乙型肝炎患者同时采用CD4+CD25+FoxP3+设门进行检测并分析。结果采用CD4+CD25+CD127-/low设门方法检测外周血Treg与CD4+CD25+FoxP3+设门方法在正常组和患者组均呈正相关(r=0.506,P<0.05;r=0.556,P<0.01)。以CD4+CD25+CD127-/low设门检测慢性乙型肝炎患者组、HBV携带者组、非活动性HBsAg携带者组和正常对照组外周血Treg的水平分别为(10.65±2.86)%、(8.56±2.01)%、(8.75±3.04)%和(7.33±1.17)%。慢性乙型肝炎患者组明显高于其他三组(P<0.01),慢性HBV携带者组明显高于对照组(P<0.05),非活动性HBsAg携带者组与对照组之间差异无统计学意义。慢性乙型肝炎患者及慢性HBV携带者Treg表达水平与HBV病毒载量之间均无相关,慢性乙型肝炎患者Treg表达水平与ALT之间无相关,HBeAg阳性患者组和HBeAg阴性患者组的Treg表达水平差异无统计学意义。结论 CD4+CD25+CD127-/low设门方法检测外周血Treg与CD4+CD25+FoxP3+设门方法有较好的相关性,而前者更为简单、实用。Treg在慢性乙型肝炎患者、慢性HBV携带者中明显增高,提示Treg在乙型肝炎慢性化机制中发挥重要作用。Objective To compare the effects of CD4^＋CD25^＋FoxP3^＋ and CD4^＋CD25^＋CD127^-/low gating on CD4^＋CD25^＋Treg and explore the expression and dinical significance of CD4^＋CD25^＋Treg in patients with chronic hepatitis B virus infection.Methods The frequency of CD4^＋CD25^＋CD127^-/low Treg in the peripheral blood from 52 chronic hepatitis B patients,24 chronic asymptomatic HBV carriers,24 inactive HBsAg carriers and 25 healthy controls were measured by flow cytometry with CD4^＋CD25^＋CD127^-/low gating.Furthermore,healthy controls and 21 chronic hepatitis B were measured by flow cytometry with CD4^＋CD25^＋FoxP3^＋.HBV DNA levels were measured by real-time PCR.Results There was a significant positive correlation between the results of CD4^＋CD25^＋CD127^-/low gating and CD4^＋CD25^＋FoxP3^＋ gating（r=0.506,P0.05;r=0.556,P0.01）.There was significant difference in the frequency of CD4^＋CD25^＋CD127^-/lowTreg between chronic hepatitis B patients（10.65±2.86）and the other groups（P0.01）.The level of Treg in chronic asymptomatic HBV carriers（8.56±2.01）was higher than that in healthy controls（7.33±1.17）.There was no correlation between serum HBV DNA levels and the frequency of Treg in chronic hepatitis B patients and chronic asymptomatic HBV carriers;No statistical was difference in the frequency of Treg between chronic hepatitis B patients of HBeAg positive and negative.Conclusion It suggested that there was a significant positive correlation between the results of CD4^＋CD25^＋CD127^-/low gating and CD4^＋CD25^＋FoxP3^＋ gating,and the former was more easily performed and practicable.The level of Treg in CHB patients and chronic asymptomatic HBV carriers is higher than that in health controls.The finding suggested that Treg plays an important role in chronic hepatitis B virus infection.

关 键 词：CD4+CD25+调节性T细胞 CD127 FOXP3 慢性HBV感染 

分 类 号：R512.62[医药卫生—内科学]