白细胞介素-33的测定及其与早期类风湿关节炎相关性的研究  

The detection of interleukin-33 and the correlated research of interleukin-33 and early rheumatoid arthritis

作　　者：黄鹤卿[1] 穆荣[2] 刘霞[2] 王世瑶[2] 李忆农[1] 栗占国[2] 

机构地区：[1]福建医科大学福州总医院风湿免疫科,350025 [2]北京大学人民医院风湿免疫科

出　　处：《中华风湿病学杂志》2010年第7期446-449,共4页Chinese Journal of Rheumatology

基　　金：国家重点基础研究发展计划（973计划）项目（2010CB529100）;国家自然科学基金（30972710）

摘　　要：目的通过检测早期类风湿关节炎（RA）患者血清中的白细胞介素（IL）-33水平，分析其与早期RA之间的相关性。方法收集病程〈1年的早期RA患者100例，骨关节炎（OA）患者40例以及健康对照者70名。采用双抗体夹心酶联免疫吸附试验（ELISA）测定血清中的IL-33水平，并分析血清IL-33水平与RA各临床和实验室指标的相关性。计量资料的比较采用Kruskal—Wallis检验和（或）Mann—Whitney U检验，计数资料比较采用矿检验，相关性分析采用Spearman相关分析。结果RA患者血清IL-33水平为（282±871）pg／ml，显著高于健康对照组[（7±38）pg／ml，P〈0．01]和OA患者[（8±35）pg／ml，P〈0．01]。血清IL-33水平与类风湿因子（RV）、隐性类风湿因子IgG（HRF—IgG）、抗环瓜氨酸肽（CCP）抗体、抗突变型瓜氨酸化波形蛋白（MCV）抗体呈正相关（r分别为：0．312，0．277，0．213，0．302，P〈0．01或P〈0．05）。IL-33阳性组患者的RF阳性率、HRF—IgG阳性率、抗CCP抗体阳性率、抗MCV抗体阳性率（86％、31％、86％、94％）较IL-33阴性组患者（54％、11％、42％、72％）显著升高（P均〈0．05）。结论IL-33在RA患者血清中显著升高，并与多种自身抗体（包括RF、抗CCP抗体、抗MCV抗体和HRF—IgG）显著相关。可能是RA预后不良的因素之一。Objective To analyze the correlation between serum interleukin （IL）-33 and early rheumatoid arthritis （RA） by testing the serum IL-33 of patients with RA. Methods One hundred patients with early RA＇whose disease duration was less than 1 year were selected, 40 patients with osteoarthritis （OA） and 70 healthy controls were selected. The serum IL-33 levels of the three groups were detected by enzyme-linked immunosorbent assay （ELISA）. The correlation between serum IL-33 levels and clinical features of patients with HA were analyzed. Kruskal-Wallis test, Mann-Whitney U test, X2 test and Spearman correlation analysis were used for statistical analysis. Results The level of serum IL-33 in RA patients [（282±871 ） pg/ml] was significantly higher than that of the healthy controls [ （7±38） pg/ml, P〈0.01 ] and OA patients [ （8±35） pg/ml, P〈0.01]. The levels of serum IL-33 in RA patients were related with rheumatoid factor （RF）, occult rheumatoid factor immunoglobnlin G （HRF-IgG）, anti-cyclic citrullinated peptide antibodies （anti-CCP）, anti-mutated citrullinated vimentin antibodies （anti-MCV）. The positive rate of RF, HRF-IgG, anti-CCP and anti-MCV in IL-33 positive group （86%, 31%, 86%, 94%） was significantly higher than that of the IL-33 negative group （54%, 11%, 42%, 72%） （P〈0.05）. Conclusion The level of serum IL-33 is significantly elevated in RA and it is significantly associated with some of the auto-antibodies （including RF, anti-CCP antibodies, anti-MCV antibodies and HRF-IgG）. Thus, IL-33 may be a factor for unfavorable prognosis to RA patients.

关 键 词：关节炎 类风湿 白细胞介素类 自身抗体 

分 类 号：R593.22[医药卫生—内科学]