有氧耐力训练对增龄大鼠骨骼肌线粒体生物合成的影响  

Effect of Aerobic Endurance Training on Mitochondrial Biogenesis of Skeletal Muscle in Aging Rats

作　　者：韩雨梅[1,2] 刘子泉[1] 常永霞[1] 刘树森[3,1] 吉力立[4,1] 张勇[1] 

机构地区：[1]天津体育学院天津市运动生理学与运动医学重点实验室,天津300381 [2]山西大学体育学院 [3]中科院动物研究所生物膜与膜生物工程国家重点实验室 [4]美国威斯康辛大学运动学系

出　　处：《中国运动医学杂志》2010年第4期425-429,共5页Chinese Journal of Sports Medicine

基　　金：天津市社会发展重大科技攻关项目(05YFGDSF02100);国家自然科学基金(30470837)

摘　　要：目的:观察增龄大鼠骨骼肌线粒体生物合成的变化特点,探讨有氧耐力训练诱导增龄大鼠骨骼肌线粒体生物合成的分子机理。方法:中等强度跑台运动(64%VO2max,5°,15m/min,45min,每周5天)施加于2、12和17月龄雄性大鼠共12周,对照组正常饲养。12周后取大鼠比目鱼肌和股外侧深层肌进行分子生物学指标检测。结果:(1)增龄过程中,PGC-1α和NRF-1 mRNA表达、mtTFA蛋白表达随增龄显著增加,而mtTFA和COXIV mRNA表达、PGC-1α和COXIV蛋白表达随增龄无显著变化。(2)耐力训练后,5MT组PGC-1α mRNA和蛋白表达、15MT组PGC-1α mRNA表达显著高于各自对照组,5MT和15MT组NRF-1 mRNA表达分别显著高于各自对照组,20MT组PGC-1α mRNA和蛋白表达、NRF-1 mRNA表达相对于20MC组无显著变化,各月龄训练组mtTFA mRNA表达、COXIV mRNA和蛋白表达均显著高于各自对照组。结论:(1)增龄过程中PGC-1α、NRF-1、mtTFA和COXIV mRNA和蛋白水平的变化呈现非同步趋势,提示增龄过程中线粒体生物合成受复杂的多条途径调控;(2)耐力训练能够诱导PGC-1α、NRF-1、mtTFA和COXIV表达显著增加,促进骨骼肌线粒体生物合成;(3)耐力训练诱导骨骼肌线粒体生物合成的适应性积累效应随着大鼠年龄递增逐渐递减。Objective The purposes of this study were to investigate the metabolic feature of mitochondrial biogenesis and to implicate the possible molecular mechanism of exercise-induced mitochondrial biogenesis in aging.Methods The moderate endurance training in a treadmill（64%VO2max,5°,15m/min,45 min,once per day for 5 days） was imposed on rats aged 2 month,12 month and 17 month for 12 wk,respectively.The control rats remained sedentary.24 hours after the last training,the rats were anesthetized and the soleuses and the deep region of the vastus lateralises were rapidly excised to test the mitochondrial protein and transcription factor expression in mitochondrial biogenesis.Results（1） The PGC-1α and NRF-1 mRNA expression along with the mtTFA protein expression were increased significantly with aging,however,both the mtTFA and COXIV mRNA expression and the PGC-1α and COXIV protein expression remained unchanged.（2） After the endurance training,the mRNA and protein expression of PGC-1α in 5MT group,also with the PGC-1α mRNA expression in 15MT group were increased markedly compared with that in 5MC and 15MC group,the same as the NRF-1 mRNA expression in 5MT and 15MT group,however,the mRNA and protein expression of PGC-1α and the NRF-1 mRNA expression in 20MT group were unchanged compared with that in 20MC group,furthermore,the mRNA and protein expression of mtTFA and COX IV in trained groups were all enhanced significantly compared with the control groups,respectively.Conclusion（1）The different metabolic feature among with the PGC-1α,NRF-1,mtTFA and COX IV at the mRNA and protein levels with aging,suggesting that complex pathways might be involved in the regulation of muscle mitochondrial biogenesis.（2） Endurance training could enhance the skeletal muscle mitochondrial biogenesis through the mRNA and protein expression increase of PGC-1α,NRF-1,mtTFA and COXIV.（3）The active effects of endurance training to induce mitochondrial biogenesis gradually decreased with the aging.

关 键 词：耐力训练 增龄 骨骼肌 线粒体生物合成 

分 类 号：R87[医药卫生—运动医学]