乔松素对脂多糖诱导的人脐静脉内皮细胞凋亡的影响  

Effect of Pinocembrin on Apoptosis in Human Umbilical Vein Endothelial Cells Induced by Lipopolysaccharide

作　　者：李伟[1] 李东娟[1] 杨艳[1] 

机构地区：[1]山东省泰山医学院,山东省泰安市271000

出　　处：《中国动脉硬化杂志》2010年第5期351-354,共4页Chinese Journal of Arteriosclerosis

基　　金：山东省自然科学基金(Y2008C86)

摘　　要：目的探讨蜂胶乔松素对脂多糖诱导的人脐静脉内皮细胞凋亡的影响。方法用消化灌注法收集人脐静脉内皮细胞进行体外原代培养,于培养液中给予10 mg/L脂多糖处理以建立细胞凋亡模型,乔松素处理组于模型条件培养液中分别加入不同浓度的乔松素(50、100和200 mg/L)干预,共同孵育24 h。光镜下观察细胞形态,MTT法测定细胞存活率以观察细胞增殖变化,缺口末端标记技术TUNEL法检测细胞凋亡率,免疫细胞化学法检测信号转导系统核因子κB亚基p65核易位变化。结果模型组内皮细胞凋亡率明显高于阴性对照组,不同浓度乔松素组细胞凋亡率均低于模型组,同时细胞存活率增高(P<0.01)。加入不同浓度乔松素能显著降低脂多糖诱导的人脐静脉内皮细胞核因子κB p65核易位活性(P<0.01)。结论乔松素可通过抑制脂多糖诱导的人脐静脉内皮细胞凋亡而保护内皮细胞功能,其机制可能与抑制核因子κB p65活化有关。Aim To investigate the effect of pinocembrin on apoptosis of human umbilical vein endothelial cells（HUVEC） induced by lipopolysaccharide（LPS）. Methods HUVEC were collected by digestion-perfusion culture.An apoptotic model of HUVEC was established by 10 mg/L LPS.The apoptosis of HUVEC induced by LPS was mixed with culture medium of 50 mg/L,100 mg/L and 200 mg/L pinocembrin,after treatment of 24 hours,HUVEC apoptotic rate was detected by TUNEL assay.Cell-morphosis was observed by light microscope.Cell survival rate was measured by MTT assay.Expression of nuclear factor–kappa B p65（NF-κB p65） was detected by immunocytochemistry staining. Results HUVEC apoptotic rate of model group increased obviously than control group,and different dosage pinocembrin group discreased significantly than model group（P0.01）.Meanwhile,cell survival rate was increased after pinocembrin treatment.Expression of NF-κB p65 in LPS induced HUVEC was reduced significantly after pinocembrin treatment compared with those induced apoptosis group by LPS（P0.01）. Conclusion Pinocembrin may inhibit the apoptosis of HUVEC,the protection mechanism of pinocembrin on HUVEC may be related to reducing the activity of NF-κB.Thereby it plays a role in protecting the vascular endothelial cells.

关 键 词：乔松素 人脐静脉内皮细胞 凋亡 脂多糖 

分 类 号：R96[医药卫生—药理学]