高血脂患者血清血管生成素样蛋白3水平测定及临床意义  

Clinical Significance of Detecting Serum Angiopioetin-Like Protein 3 in Patients with Hyperlipoidemia

作　　者：李晞[1,2] 王继红[1,2] 肖亚雄[1,2] 王永红[3] 

机构地区：[1]重庆医科大学基础医学实验教学中心生物化学与分子生物学实验室,重庆市400016 [2]重庆医科大学分子医学与肿瘤研究中心,重庆市400016 [3]重庆医科大学附属第一医院体检中心,重庆市400016

出　　处：《中国动脉硬化杂志》2010年第5期389-391,共3页Chinese Journal of Arteriosclerosis

基　　金：重庆市科委自然科学基金(CSTC;2008CE9142);重庆医科大学创新基金(CX200528)

摘　　要：目的应用ELISA双抗夹心法测定高血脂患者血清血管生成素样蛋白3水平,探讨其对检测脂质代谢紊乱的临床价值。方法通过检测高甘油三酯血症、高总胆固醇血症和甘油三酯与胆固醇两者均升高3组高脂血症患者血清中血管生成素样蛋白3含量,并与正常对照组比较,分析其检测的临床意义。结果单纯高甘油三酯血症患者血清血管生成素样蛋白3含量与正常对照组比较差异有显著性(P<0.05),且与临床诊断高甘油三酯血症的常用方法GOD-POD法比较,诊断效率差异无显著性(P>0.05)。单纯高总胆固醇血症患者血清血管生成素样蛋白3含量与正常对照组比较差异无显著性(P>0.05)。总胆固醇和甘油三酯均升高患者血清血管生成素样蛋白3含量与对照组,单纯高甘油三酯血症和单纯高总胆固醇血症差异无显著性(P>0.05)。结论 ELISA方法可以灵敏地检测到人血清中血管生成素样蛋白3含量,在单纯高甘油三酯血症时明显增加,而在单纯高胆固醇血症时,患者血清血管生成素样蛋白3含量变化不明显,为建立人血清血管生成素样蛋白3检测的可行性奠定了基础。Aim To explore the clinical value of the content of Angiopioetin-like protein 3（ANGPTL3）in patients with hyperlipoidemia by ELISA. Methods Serum ANGPTL3 was examined by ELISA in the patients with hypercholesterolemia（HTC）,hypertriglyceridemia（HTG） and patients with both of them,and compared with normal group. Results In comparison with the normal group,the content of serum ANGPTL3 of patients with HTG was increased significantly（P0,05）,and there was no significant difference in diagnosis of HTG between detecting ANGPTL3 by ELISA and TG by GOD-POD method.But the content of serum ANGPTL3 of patients with HTC made no difference with normal group（P0.05）.And variance in the contents of serum ANGPTL3 of patients with both of HTC and HTG was not significant. Conclusion ELISA method can detect the increase in the patients with HTG,and there was no difference in the patients with HTC sensitively,which laid a foundation for application of ANGPTL3 ELISA kit in clinical laboratory.

关 键 词：血管生成素样蛋白3 酶联免疫吸附法 高甘油三酯血症 高总胆固醇血症 

分 类 号：Q54[生物学—生物化学]