子宫内膜癌与胰岛素-Ras-MAPK信号通路的相关性研究  

Correlation Study of Endometrial Carcinoma and Insulin-Ras-MAPK Signaling Pathway

作　　者：田文艳[1] 窦文婷[1] 王颖梅[1] 张丽志[1] 薛凤霞[1] 张灏[2] 

机构地区：[1]天津医科大学总医院妇产科,300052 [2]天津医科大学基础医学院细胞生物学实验室

出　　处：《天津医药》2010年第7期545-548,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30772316)

摘　　要：目的:探讨胰岛素-Ras-MAPK通路中主要调节蛋白生长因子受体结合蛋白2(Grb-2)、Ras、P-ERK、细胞周期素D1(CD-1)在子宫内膜癌中的表达及意义。方法:采用免疫组化方法检测48例正常子宫内膜,10例非典型增生子宫内膜,51例子宫内膜癌组织中Grb-2、Ras、P-ERK及CD-1的表达情况,放免法检测内膜癌患者空腹血清胰岛素水平。结果:Grb-2、Ras、P-ERK、CD-1在正常子宫内膜组织、非典型增生子宫内膜、子宫内膜癌组织中阳性表达率依次升高,其中Grb-2、P-ERK、CD-1差别有统计学意义。不同病理分期的子宫内膜癌患者组织中Grb-2阳性表达情况比较差异有统计学意义,P-ERK阳性表达差异亦有统计学意义(均P<0.01),子宫内膜癌中Grb-2与Ras、Grb-2与P-ERK表达呈正相关,差异有统计学意义(P<0.05),在有无淋巴结转移的子宫内膜癌中Grb-2的表达差异有统计学意义有关(P<0.05)。血胰岛素水平与Grb-2、P-ERK表达呈正相关(P<0.05)。结论:胰岛素-Ras-MAPK信号传导通路在子宫内膜癌的发生发展过程中可能起重要作用。Objective：To investigate the expression and correlation of receptor-bound protein-2（Grb-2）,Ras,P-ERK and cyclin D1（CD-1）,which are the main members of insulin-Ras-MAPK signal pathway,in endometrial carcinoma.Methods：The expressions of Grb-2,Ras,P-ERK and CD-1 protein were detected by using immunohistochemistry Elivison method and image analysis system in tissues from 51 cases of endometrial carcinoma,10 endometrial atypical hyperplasia,and 48 normal endometrium.The serum insulin concentration was measured by radioimmunoscintigraphy in endometrial carcinoma.Results：The positive expression rates of Grb-2,Ras,P-ERK and CD-1 were gradually increased in normal endometrium,endometrial atypical hyperplasia and endometrial carcinoma.The expression rates of Grb-2,Ras,P-ERK and CD-1 were higher in endometrial carcinoma than those in normal endometrium and endometrial atypical hyperplasia.In endometrial carcinoma there was a positive correlation between Grb-2 and FIGO stage,and between P-ERK and FIGO stage（P 〈0.05）.Also,there was a positive correlation between Grb-2 and lymphonode metastasis（P 〈0.05）.There were positive correlations between the expression rates of Grb-2-Ras,Grb-2-P-ERK in endometrial carcinoma.The expression of Grb-2 and P-ERK was correlated with fasting serum insulin level in endometrial carcinoma（P 〈0.05）.Conclusion：Insulin-Ras-MAPK pathway may play an important role in the carcinogenesis and development of endometrial cancer.

关 键 词：子宫内膜肿瘤 胰岛素 基因 ras MAP激酶信号系统 GRB2衔接蛋白质 丝裂原激活蛋白激酶类细胞周期蛋白D1 免疫组织化学 

分 类 号：R587[医药卫生—内分泌]